通过灵敏的酶联免疫吸附测定法定量检测人补体的C3d裂解产物。

Quantification of the C3d split products of human complement by a sensitive enzyme-linked immunosorbent assay.

作者信息

Mollnes T E

出版信息

Scand J Immunol. 1985 Jun;21(6):607-13. doi: 10.1111/j.1365-3083.1985.tb01851.x.

DOI:10.1111/j.1365-3083.1985.tb01851.x

Abstract

A double-antibody biotin-avidin enzyme-linked immunosorbent assay (ELISA) for quantification of the C3d split products is described. Polyethylene glycol 6000 was used to precipitate large C3 molecules, and the C3d-containing supernatant was used in the assay. C3d was measured in ethylenediaminetetraacetic acid plasma from 40 healthy blood donors, and the normal range was defined. Twenty-two patients were tested, and 12 of these had increased levels of C3d. No correlation was observed between total C3 and C3d in these patients. There was a close correlation between C3d measured by this method and by the double-decker rocket immunoelectrophoresis method. The C3d ELISA method is very sensitive, easy to perform, and time-saving and economical compared with most C3d methods already described. A procedure to define the lower detection limit and examine the reliability of an ELISA method in general is discussed.

摘要

描述了一种用于定量C3d裂解产物的双抗体生物素-抗生物素蛋白酶联免疫吸附测定（ELISA）。使用聚乙二醇6000沉淀大的C3分子，并将含C3d的上清液用于该测定。对40名健康献血者的乙二胺四乙酸血浆中的C3d进行了测量，并确定了正常范围。对22名患者进行了检测，其中12名患者的C3d水平升高。在这些患者中，未观察到总C3与C3d之间的相关性。通过该方法测量的C3d与双层火箭免疫电泳法测量的C3d之间存在密切相关性。与已描述的大多数C3d方法相比，C3d ELISA方法非常灵敏、易于操作，且省时经济。讨论了确定下限检测限和一般检查ELISA方法可靠性的程序。