Clin Chem Lab Med. 2014 May;52(5):693-9. doi: 10.1515/cclm-2013-0676.
In the Retic channel of DxH 800 (Beckman Coulter), the red blood cells (RBCs) resistant to hemoglobin clearing are counted as unghosted cells (UGCs). The aim of this study was to evaluate that the UGC is a surrogate marker for both the detection and counting of target cells.
In total, 1181 samples including 22 from iron deficiency anemia (IDA) patients, 95 from jaundice, 2 from sickle cell anemia, 3 from thalassemia, 1 cord blood, and 269 from normal controls were analyzed. Slides were prepared from all samples except normal controls and target cells were counted for correlation analysis of target cell counts to UGCs.
The normal control samples showed 0.01% (0%-0.01%) UGCs, and the reference range was set at ≤0.02%. The IDA samples showed 0.015% (0.01%-0.03%) UGC count and 0.05% (0%-0.2%) target cell count. The jaundice samples showed 0.98% (0.1%-5.36%) UGC count, and 1.4% (0.1%-7.0%) target cell count. The two sickle cell anemia samples showed 0.41% and 3.74% UGC counts and 0.4% and 11.5% target cell counts. A cord blood sample showed 0.01% UGCs and 0% target cells. The three thalassemia samples showed 0.01%, 1.99%, and 7.82% UGC counts and 0%, 1.4%, and 15.5% target cell counts. The samples showing poikilocytosis other than target cells showed normal UGC count (≤0.02%). The positive predictive value of UGCs was 58.2% (124/213) and the negative predictive value was 96.8% (674/696). The UGC counts were well correlated to the manual target cell counts (r=0.944, p=0.000).
This study demonstrates for the first time in the literature that a hematological parameter obtained automatically every time a reticulocyte counting is performed can be used to both screen for the presence of target cells and reliably quantify them.
在 DxH 800（贝克曼库尔特）的 Retic 通道中，对血红蛋白清除有抗性的红细胞（RBC）被计为未去影细胞（UGC）。本研究旨在评估 UGC 是否可作为检测和计数靶细胞的替代标志物。
共分析了 1181 个样本，包括 22 个缺铁性贫血（IDA）患者样本、95 个黄疸样本、2 个镰状细胞贫血样本、3 个地中海贫血样本、1 个脐血样本和 269 个正常对照样本。除正常对照样本外，所有样本均制备载玻片并进行靶细胞计数，以分析靶细胞计数与 UGC 的相关性。
正常对照样本的 UGC 计数为 0.01%（0%-0.01%），参考范围设定为≤0.02%。IDA 样本的 UGC 计数为 0.015%（0.01%-0.03%），靶细胞计数为 0.05%（0%-0.2%）。黄疸样本的 UGC 计数为 0.98%（0.1%-5.36%），靶细胞计数为 1.4%（0.1%-7.0%）。两个镰状细胞贫血样本的 UGC 计数分别为 0.41%和 3.74%，靶细胞计数分别为 0.4%和 11.5%。一个脐血样本的 UGC 计数为 0.01%，靶细胞计数为 0%。三个地中海贫血样本的 UGC 计数分别为 0.01%、1.99%和 7.82%，靶细胞计数分别为 0%、1.4%和 15.5%。除靶细胞外，其他异形红细胞样本的 UGC 计数正常（≤0.02%）。UGC 的阳性预测值为 58.2%（124/213），阴性预测值为 96.8%（674/696）。UGC 计数与手动靶细胞计数高度相关（r=0.944，p=0.000）。
本研究首次在文献中证明，每次进行网织红细胞计数时自动获得的血液学参数可用于筛查靶细胞的存在并可靠地定量靶细胞。
