Department of Botany, North Carolina State University, 27650, Raleigh, NC, USA.
Planta. 1979 Dec;147(2):156-8. doi: 10.1007/BF00389517.
The numbers of embryogenic (S) grains present in in-situ mature anthers of Nicotiana tabacum L. were compared to the numbers of embryos and plantlets produced in cultured anthers excised at the optimal mitotic stage of development for anther culture. The Feulgen technique of staining embryos caused a considerable loss of grains from cultured anthers but this did not seriously affect the determination of the percentage of embryos present. In no instance did the numbers of embryos produced exceed the maximum number of S grains found, and the distributions of S grain and embryo frequencies in anthers were similar. In rare instances S grains which had undergone the first embryogenic division were observed in situ. The results indicate that all grains capable of embryogenesis are determined during early flower formation and that their number is not increased by in vitro culture.
对烟草原生成熟花药中胚性（S）颗粒的数量与在离体培养的花药中最佳有丝分裂阶段切除的花药中胚胎和植物的数量进行了比较。胚胎的Feulgen 染色技术导致培养的花药中的颗粒大量丢失，但这并没有严重影响胚胎存在的百分比的确定。在任何情况下，胚胎的数量都不会超过发现的 S 颗粒的最大数量，并且花药中 S 颗粒和胚胎频率的分布相似。在极少数情况下，观察到了在原位进行第一次胚性分裂的 S 颗粒。结果表明，所有有能力进行胚胎发生的颗粒都在早期花形成过程中确定，并且其数量不会通过体外培养增加。
