Botanisches Institut der Universität, Gyrhofstraße 15, D-5000, Köln 41, Germany.
Planta. 1979 Dec;147(2):159-62. doi: 10.1007/BF00389518.
The uptake of sucrose against a concentration gradient into the dextran-impermeable [(3)H]H2O space of red beet (Beta vulgaris L.) vacuoles has been studied using silicone-layer-filtering centrifugation on both fluorometric and (14)C-measurement of sucrose. Sucrose transport into vacuoles proceeds partly by an active transport system and partly by passive permeation. The K M(20°C) for active sucrose uptake was found to be about 22 mM and the V Max(20°C) was about 174 nmol sucrose x (unit betacyanin)(-1) x h(-1). The temperature dependency of sucrose transport appears to have an activation energy of 35,0 KJ×mol(-1). Among various mono-, di-, and trisaccharides tested, raffinose acts as a competitive inhibitor of sucrose uptake.
采用硅酮层过滤离心法，在荧光计和 14C 测量蔗糖的条件下，研究了蔗糖在浓度梯度下进入甜菜（Beta vulgaris L.）液泡的葡聚糖不可渗透 [(3)H]H2O 空间的摄取情况。蔗糖进入液泡的部分是通过主动运输系统进行的，部分是通过被动渗透进行的。在 20°C 下，发现主动蔗糖摄取的 K M 值约为 22mM，V Max 值约为 174nmol 蔗糖 x（单位甜菜红素）-1 x h-1。蔗糖运输的温度依赖性似乎具有 35.0KJ×mol-1 的活化能。在所测试的各种单糖、二糖和三糖中，棉子糖作为蔗糖摄取的竞争性抑制剂起作用。
