Thankamani V, Kumari T V, Vasudevan D M
J Exp Pathol. 1985 Summer;2(2):123-33.
Indirect immunofluorescence technique was employed to detect herpes simplex virus type-2 (HSV-2) antigens in tumor biopsies from 215 patients with carcinoma of the uterine cervix. A total of 169 samples (79%) revealed brilliant nuclear fluorescence. Inflammatory cells infiltrating the tumor mass were positive to 60 of the 215 patients (28%). Samples showed no significant variation in the degree of fluorescence or proportion of cells binding HSV antibody with advancement in the clinical stage of the disease. Fluorescence was totally abolished when incubated with HSV-2 antiserum absorbed with a specific homologous virus. Among controls, there was fluorescence in 27% of cervical scrapings from normal women and 34% (42/124) among patients with gynecological disorders other than cervical malignancy. In cervical dysplasia 23 out of 40 patients (58%) expressed herpes virus-associated antigens. There was membrane fluorescence in live malignant cell preparations in 3 of 28 patients (11%). Normal cervix tissue from hysterectomy specimens and breast cancer cells were negative for herpes simplex virus-related antigens. Pre-immune serum and PBS showed nonspecific fluorescence in 25% and 23% of sera, respectively.
采用间接免疫荧光技术检测215例子宫颈癌患者肿瘤活检组织中的单纯疱疹病毒2型（HSV - 2）抗原。总共169份样本（79%）显示出明亮的核荧光。浸润肿瘤块的炎性细胞在215例患者中有60例呈阳性（28%）。样本在荧光强度或与HSV抗体结合的细胞比例方面，并未随疾病临床分期的进展而出现显著变化。当与用特定同源病毒吸收的HSV - 2抗血清孵育时，荧光完全消失。在对照组中，正常女性宫颈刮片中27%有荧光，除宫颈恶性肿瘤外的妇科疾病患者中有34%（42/124）有荧光。在宫颈发育异常患者中，40例中有23例（58%）表达疱疹病毒相关抗原。在28例患者的活体恶性细胞制剂中有3例（11%）出现膜荧光。子宫切除标本的正常宫颈组织和乳腺癌细胞中单纯疱疹病毒相关抗原呈阴性。免疫前血清和PBS分别在25%和23%的血清中显示非特异性荧光。
