Institute of Diagnostic and Interventional Radiology, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China,
Radiol Med. 2014 Apr;119(4):269-76. doi: 10.1007/s11547-013-0335-2. Epub 2013 Dec 6.
Malignant tumours develop strategies to avoid immune recognition and elimination by T cells, even in individuals with a fully functioning immune system. To explore the treatment approach of adoptive immunotherapy, we exploited T cells loaded with radiolabelled gold nanoparticles (AuNPs) to track T cells in vivo.
Surface-modified AuNPs were radiolabelled with (111)In or (64)Cu. They were then transferred into T cells via electroporation. To evaluate the effectiveness of this process, T cells loaded with (111)In-radiolabelled AuNPs were injected directly into the right lung of nude mice for in vivo imaging by micro-SPECT/CT. T cells loaded with (64)Cu-radiolabelled AuNPs were then injected into the tail vein of nude mice and imaged by micro-PET/CT.
High uptake signals were observed in the right lung following the direct injection of T cells containing (111)In-labelled AuNPs. Imaging showed a marked difference in the dynamic biodistribution of T cells containing (64)Cu-labelled AuNPs when compared with (64)Cu-labelled AuNPs alone.
This study demonstrated the feasibility of the in vivo imaging of T cells loaded with radiolabelled AuNPs.
恶性肿瘤会发展出各种策略来逃避 T 细胞的免疫识别和清除，即使在免疫系统功能正常的个体中也是如此。为了探索过继免疫疗法的治疗方法，我们利用负载放射性标记金纳米颗粒（AuNPs）的 T 细胞来追踪体内的 T 细胞。
对 AuNPs 进行表面修饰，并进行（111）In 或（64）Cu 放射性标记。然后通过电穿孔将其转入 T 细胞。为了评估这一过程的有效性，将负载（111）In 放射性标记 AuNPs 的 T 细胞直接注入裸鼠的右肺，通过 micro-SPECT/CT 进行体内成像。然后将负载（64）Cu 放射性标记 AuNPs 的 T 细胞注入裸鼠尾静脉，并通过 micro-PET/CT 进行成像。
直接注入负载（111）In 标记 AuNPs 的 T 细胞后，在右肺中观察到高摄取信号。与单独的（64）Cu 标记 AuNPs 相比，（64）Cu 标记 AuNPs 负载的 T 细胞的动态生物分布有明显差异。
本研究证明了负载放射性标记 AuNPs 的 T 细胞体内成像的可行性。
