In vivo imaging of T cells loaded with gold nanoparticles: a pilot study.

PURPOSE

Malignant tumours develop strategies to avoid immune recognition and elimination by T cells, even in individuals with a fully functioning immune system. To explore the treatment approach of adoptive immunotherapy, we exploited T cells loaded with radiolabelled gold nanoparticles (AuNPs) to track T cells in vivo.

MATERIALS AND METHODS

Surface-modified AuNPs were radiolabelled with (111)In or (64)Cu. They were then transferred into T cells via electroporation. To evaluate the effectiveness of this process, T cells loaded with (111)In-radiolabelled AuNPs were injected directly into the right lung of nude mice for in vivo imaging by micro-SPECT/CT. T cells loaded with (64)Cu-radiolabelled AuNPs were then injected into the tail vein of nude mice and imaged by micro-PET/CT.

RESULTS

High uptake signals were observed in the right lung following the direct injection of T cells containing (111)In-labelled AuNPs. Imaging showed a marked difference in the dynamic biodistribution of T cells containing (64)Cu-labelled AuNPs when compared with (64)Cu-labelled AuNPs alone.

CONCLUSIONS

This study demonstrated the feasibility of the in vivo imaging of T cells loaded with radiolabelled AuNPs.