Perry R R, Bass B L, Harmon J W, Garcia V F
J Surg Res. 1986 Dec;41(6):627-35. doi: 10.1016/0022-4804(86)90088-0.
Avascular fetal rat intestine becomes vascularized and grows and develops certain properties of normal small intestine when transplanted into the subcutaneous tissues of host syngeneic rats. We quantified the blood flow to this transplanted intestine (called "neogut") to clarify the role of angiogenesis in its growth and development. Additionally, we correlated blood flow and the growth of neogut into either patent tubular segments or large saccular segments with associated strictures. Blood flow was studied using 141Ce or 85Sr labeled microspheres. Blood flows are expressed as mean +/- SEM in (ml/min X g). Neogut blood flow (0.19 +/- 0.02) was greater than that of adjacent subcutaneous tissue (0.03 +/- 0.00, P less than 0.01) but less than that of native small bowel (0.85 +/- 0.07, P less than 0.01). The blood flow to the large saccular segments (0.10 +/- 0.01) was less than that to the tubular portions (0.27 +/- 0.02, P less than 0.01). The weight of neogut was comparable to that of native small bowel. These findings demonstrate that neogut implants stimulate angiogenesis to a degree which may be sufficient for it to absorb nutrients. Furthermore, ischemia is present in large saccular neogut segments with associated strictures. Additional investigation appears warranted to see if neogut can serve as a clinically useful small bowel substitute.
将无血管的胎鼠小肠移植到同基因宿主大鼠的皮下组织后，它会血管化，并生长发育出正常小肠的某些特性。我们对移植到此处的小肠（称为“新肠”）的血流量进行了量化，以阐明血管生成在其生长发育中的作用。此外，我们将血流量与新肠生长为有腔管状段或伴有狭窄的大囊状段的情况进行了关联分析。使用141Ce或85Sr标记的微球研究血流量。血流量以（ml/min×g）表示为平均值±标准误。新肠血流量（0.19±0.02）高于相邻皮下组织（0.03±0.00，P<0.01），但低于天然小肠（0.85±0.07，P<0.01）。大囊状段的血流量（0.10±0.01）低于管状部分（0.27±0.02，P<0.01）。新肠的重量与天然小肠相当。这些发现表明，新肠植入物能在一定程度上刺激血管生成，这可能足以使其吸收营养。此外，伴有狭窄的大囊状新肠段存在缺血情况。似乎有必要进行进一步研究，以确定新肠是否可作为临床上有用的小肠替代物。
