Bass B L, Schweitzer E J, Harmon J W, Tai Y H, Sjogren R W, Kraimer J
Ann Surg. 1984 Dec;200(6):734-41. doi: 10.1097/00000658-198412000-00011.
Avascular segments of fetal rat intestine transplanted to the subcutaneous tissues of host syngeneic rats will become vascularized and grow. This study more fully characterizes this tissue, which we call "neogut," and compares it to normal rat small intestine. Anatomy was studied with light microscopy and scanning and transmission electron microscopy; transport and electrophysiologic parameters were measured in full-thickness pieces of tissue mounted in Ussing chambers; motility patterns, including slow wave and spike activity, were recorded. Subtle anatomic differences (shortened villi and microvilli) were noted in neogut compared to normal small bowel. Both neogut and normal rat ileum demonstrated net mucosal to serosal transport of d-glucose; the magnitudes of the electrophysiologic parameters (PD, Isc, and G) were less in neogut than in ileum. Slow-wave frequency of neogut was slightly less than native small bowel while spike activity was increased. These data show that neogut has structural and physiologic characteristics similar to normal rat small bowel and offers hope that this tissue may provide a nutritionally useful accessory gut for the patient with critical short-gut syndrome.
将胎鼠肠道的无血管节段移植到同基因宿主大鼠的皮下组织后，这些节段会血管化并生长。本研究更全面地描述了这种我们称为“新肠”的组织，并将其与正常大鼠小肠进行比较。通过光学显微镜、扫描电子显微镜和透射电子显微镜研究解剖结构；在安装于尤斯灌流小室中的全层组织片上测量转运和电生理参数；记录包括慢波和锋电位活动在内的运动模式。与正常小肠相比，新肠存在细微的解剖学差异（绒毛和微绒毛缩短）。新肠和正常大鼠回肠均表现出葡萄糖从黏膜到浆膜的净转运；新肠的电生理参数（跨膜电位、短路电流和电导）幅度低于回肠。新肠的慢波频率略低于天然小肠，而锋电位活动增加。这些数据表明，新肠具有与正常大鼠小肠相似的结构和生理特征，并为这种组织可能为患有严重短肠综合征的患者提供营养上有用的辅助肠道带来了希望。
