Jones Bryony A, Sauter-Louis Carola, Henning Joerg, Stoll Alexander, Nielen Mirjam, Van Schaik Gerdien, Smolenaars Anja, Schouten Matthijs, den Uijl Ingrid, Fourichon Christine, Guatteo Raphael, Madouasse Aurélien, Nusinovici Simon, Deprez Piet, De Vliegher Sarne, Laureyns Jozef, Booth Richard, Cardwell Jackie M, Pfeiffer Dirk U
Department of Population & Production Health, Royal Veterinary College, North Mymms, Herts, United Kingdom.
PLoS One. 2013 Dec 2;8(12):e80619. doi: 10.1371/journal.pone.0080619. eCollection 2013.
Bovine neonatal pancytopenia (BNP), a high fatality condition causing haemorrhages in calves aged less than 4 weeks, was first reported in 2007 in Germany and subsequently observed at low incidence in other European countries and New Zealand. A multi-country matched case-control study was conducted in 2011 to identify calf-level risk factors for BNP. 405 BNP cases were recruited from 330 farms in Belgium, France, Germany and the Netherlands by laboratory confirmation of farmer-reported cases. Up to four calves of similar age from the same farm were selected as controls (1154 calves). Risk factor data were collected by questionnaire. Multivariable modelling using conditional logistic regression indicated that PregSure®BVD (PregSure, Pfizer Animal Health) vaccination of the dam was strongly associated with BNP cases (adjusted matched Odds Ratio - amOR 17.8 first lactation dams; 95% confidence interval - ci 2.4, 134.4; p = 0.005), and second or more lactation PregSure-vaccinated dams were more likely to have a case than first lactation vaccinated dams (amOR 2.2 second lactation; ci 1.1, 4.3; p = 0.024; amOR 5.3 third or more lactation; ci 2.9, 9.8; p = <0.001). Feeding colostrum from other cows was strongly associated with BNP if the dam was not PregSure-vaccinated (amOR 30.5; ci 2.1, 440.5; p = 0.012), but the effect was less if the dam was PregSure-vaccinated (amOR 2.1; ci 1.1, 4.0; p = 0.024). Feeding exclusively dam's milk was a higher risk than other types of milk (amOR 3.4; ci 1.6, 7.5; p = 0.002). The population attributable fractions were 0.84 (ci 0.68, 0.92) for PregSure vaccination, 0.13 (ci 0.06, 0.19) for feeding other cows' colostrum, and 0.15 (ci 0.08, 0.22) for feeding dam's milk. No other calf-level factors were identified, suggesting that there are other important factors that are outside the scope of this study, such as genetics, which explain why BNP develops in some PregSure-colostrum-exposed calves but not in others.
牛新生儿全血细胞减少症（BNP）是一种高致死性疾病，可导致4周龄以下犊牛出血，于2007年在德国首次报道，随后在其他欧洲国家和新西兰以低发病率被观察到。2011年进行了一项多国匹配病例对照研究，以确定犊牛水平的BNP风险因素。通过实验室对农民报告病例的确认，从比利时、法国、德国和荷兰的330个农场招募了405例BNP病例。从同一农场选择年龄相似的多达四头犊牛作为对照（1154头犊牛）。通过问卷调查收集风险因素数据。使用条件逻辑回归的多变量建模表明，对母牛进行PregSure®BVD（PregSure，辉瑞动物保健公司）疫苗接种与BNP病例密切相关（调整后的匹配优势比 - amOR，初产母牛为17.8；95%置信区间 - ci为2.4, 134.4；p = 0.005），并且第二次或更多次泌乳且接种了PregSure疫苗的母牛比初产接种疫苗的母牛更有可能出现病例（第二次泌乳amOR为2.2；ci为1.1, 4.3；p = 0.024；第三次或更多次泌乳amOR为5.3；ci为2.9, 9.8；p < 0.001）。如果母牛未接种PregSure疫苗，喂其他母牛的初乳与BNP密切相关（amOR为30.5；ci为2.1, 440.5；p = 0.012），但如果母牛接种了PregSure疫苗，这种影响较小（amOR为2.1；ci为1.1, 4.0；p = 0.024）。仅喂母牛的奶比其他类型的奶风险更高（amOR为3.4；ci为1.6, 7.5；p = 0.002）。PregSure疫苗接种的人群归因分数为0.84（ci为0.68, 0.92），喂其他母牛的初乳为0.13（ci为0.06, 0.19），喂母牛的奶为0.15（ci为0.08, 0.22）。未发现其他犊牛水平的因素，这表明存在本研究范围之外的其他重要因素，如遗传学，这解释了为什么一些接触PregSure初乳的犊牛会发生BNP而其他犊牛不会。
