Clinical Dental Research Core, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, United States of America.
PLoS One. 2013 Dec 2;8(12):e81635. doi: 10.1371/journal.pone.0081635. eCollection 2013.
Despite the important diagnostic value of evaluating antibody responses to individual human pathogens, antibody profiles against multiple infectious agents have not been used to explore health and disease mainly for technical reasons. We hypothesized that the interplay between infection and chronic disease might be revealed by profiling antibodies against multiple agents. Here, the levels of antibodies against a panel of 13 common infectious agents were evaluated with the quantitative Luciferase Immunoprecipitation Systems (LIPS) in patients from three disease cohorts including those with pathogenic anti-interferon-γ autoantibodies (IFN-γ AAB), HIV and Sjögren's syndrome (SjS) to determine if their antibody profiles differed from control subjects. The IFN-γ AAB patients compared to controls demonstrated statistically higher levels of antibodies against VZV (p=0.0003), EBV (p=0.002), CMV (p=0.003), and C. albicans (p=0.03), but lower antibody levels against poliovirus (p=0.04). Comparison of HIV patients with blood donor controls revealed that the patients had higher levels of antibodies against CMV (p=0.0008), HSV-2 (p=0.0008), EBV (p=0.001), and C. albicans (p=0.01), but showed decreased levels of antibodies against coxsackievirus B4 (p=0.0008), poliovirus (p=0.0005), and HHV-6B (p=0.002). Lastly, SjS patients had higher levels of anti-EBV antibodies (p=0.03), but lower antibody levels against several enteroviruses including a newly identified picornavirus, HCoSV-A (p=0.004), coxsackievirus B4 (p=0.04), and poliovirus (p=0.02). For the IFN-γ AAB and HIV cohorts, principal component analysis revealed unique antibody clusters that showed the potential to discriminate patients from controls. The results suggest that antibody profiles against these and likely other common infectious agents may yield insight into the interplay between exposure to infectious agents, dysbiosis, adaptive immunity and disease activity.
尽管评估针对个体人类病原体的抗体反应具有重要的诊断价值，但由于技术原因，尚未将针对多种感染因子的抗体谱用于探索健康和疾病。我们假设，通过分析针对多种病原体的抗体，可以揭示感染与慢性疾病之间的相互作用。在这里，我们使用定量 Luciferase Immunoprecipitation Systems (LIPS) 评估了来自三个疾病队列的患者对 13 种常见感染因子的抗体水平，这些队列包括具有致病性抗干扰素-γ自身抗体 (IFN-γ AAB)、HIV 和干燥综合征 (SjS) 的患者，以确定其抗体谱是否与对照不同。与对照组相比，IFN-γ AAB 患者表现出针对 VZV（p=0.0003）、EBV（p=0.002）、CMV（p=0.003）和 C. albicans（p=0.03）的抗体水平显著升高，但针对脊髓灰质炎病毒的抗体水平较低（p=0.04）。与血液供体对照相比，HIV 患者的比较表明，患者对 CMV（p=0.0008）、HSV-2（p=0.0008）、EBV（p=0.001）和 C. albicans（p=0.01）的抗体水平更高，但对柯萨奇病毒 B4（p=0.0008）、脊髓灰质炎病毒（p=0.0005）和 HHV-6B（p=0.002）的抗体水平降低。最后，SjS 患者的 EBV 抗体水平较高（p=0.03），但针对几种肠道病毒的抗体水平较低，包括新发现的小核糖核酸病毒 HCoSV-A（p=0.004）、柯萨奇病毒 B4（p=0.04）和脊髓灰质炎病毒（p=0.02）。对于 IFN-γ AAB 和 HIV 队列，主成分分析揭示了独特的抗体簇，这些抗体簇有可能将患者与对照区分开来。结果表明，针对这些以及可能其他常见感染因子的抗体谱可能有助于深入了解感染因子暴露、生态失调、适应性免疫和疾病活动之间的相互作用。
