利用薄膜法制备载塞来昔布脂质体
Utilization of thin film method for preparation of celecoxib loaded liposomes.
作者信息
Moghimipour Eskandar, Handali Somayeh
机构信息
Nanotechnology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
出版信息
Adv Pharm Bull. 2012;2(1):93-8. doi: 10.5681/apb.2012.013. Epub 2012 Apr 18.
PURPOSE
Celecoxib is nonsteroiddal anti-inflammatory drug that has been used extensively to treat patients with arthritis. The aim of the present study was to formulate and characterize liposomal vesicles loaded with celecoxib.
METHODS
Liposomes were prepared by thin film method using soya lecithin and cholesterol. The release of drug was determined using a dialysis membrane method. Liposomes were characterized by Differential Scanning Calorimetery (DSC), Transmission Electron Microscopy (TEM) and their particle size was also determined.
RESULTS
The results showed that the drug encapsulation efficiency was 67.34% and there was 67.16% release after 0.5, 1, 2, 3, 4, 5, 6, 7, 8 and 24 h. RESULTS of particle size determination showed a mean size of 677nm and nanoparticles were spherical as shown by TEM. The DSC curve of lecithin, cholesterol and celecoxib were different from celecoxib containing liposome.
CONCLUSION
The results of characterization of the vesicles indicated the potential application of celecoxib loaded liposome as carrier system.
目的
塞来昔布是一种非甾体抗炎药，已被广泛用于治疗关节炎患者。本研究的目的是制备并表征负载塞来昔布的脂质体囊泡。
方法
采用薄膜法，以大豆卵磷脂和胆固醇制备脂质体。使用透析膜法测定药物释放情况。通过差示扫描量热法（DSC）、透射电子显微镜（TEM）对脂质体进行表征，并测定其粒径。
结果
结果显示，药物包封率为67.34%，在0.5、1、2、3、4、5、6、7、8和24小时后释放率为67.16%。粒径测定结果显示平均粒径为677nm，如TEM所示纳米颗粒呈球形。卵磷脂、胆固醇和塞来昔布的DSC曲线与含塞来昔布的脂质体不同。
结论
囊泡的表征结果表明负载塞来昔布的脂质体作为载体系统具有潜在应用价值。
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