Moghimipour Eskandar, Aghel Nasrin, Zarei Mahmoudabadi Ali, Ramezani Zahra, Handali Somayeh
Nanotechnology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran ; Medicinal Plant Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
Medicinal Plant Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
Jundishapur J Nat Pharm Prod. 2012 Summer;7(3):117-22. Epub 2012 Aug 25.
The increased incidence of fungal resistance has necessitated the need to search for new antifungal agents.
The main objectives of the present study were to investigate the effectiveness of the essential oil of Eucalyptus camaldulensis on dermatophytes growth and to formulate and characterize a liposomal gel loaded with the essential oil.
The essential oil extracted from the leaves of E. camaldulensis was analyzed by GC-MS. The antifungal activity of this essential oil was determined against Microsporum canis, M. gypseum, Trichophyton rubrum and T. verrucosum, using the well diffusion method. Liposomes were prepared by the freeze-thaw method and evaluation of size distribution was performed using a particle size analyzer. The liposomal gel was prepared using 'hydroxethyl cellulose (HEC) as the gelling agent. The rheologic characteristics were determined by a Brookfield viscometer.
The results showed that the minimum inhibitory volume of the essential oil was 0.125 ml and 95 ± 0.57% of the essential oil was successfully entrapped in the liposomes. The main constituents of the essential oil detected by GC-MS were; phenol, 1, 8 cineole, limonene, alcohol, pinene and terpinen. Results of particle size determination showed a wide range from 40.5 to 298 nm for the different formulations. No significant thixotropy was observed in the rheogram of the formulated liposomal gel.
Liposomal gel formulation of the essential oil may lead to improved antifungal activity.
真菌耐药性的增加使得寻找新型抗真菌药物成为必要。
本研究的主要目的是研究赤桉精油对皮肤癣菌生长的有效性，并制备和表征负载该精油的脂质体凝胶。
采用气相色谱-质谱联用仪（GC-MS）分析从赤桉叶中提取的精油。采用打孔扩散法测定该精油对犬小孢子菌、石膏样小孢子菌、红色毛癣菌和疣状毛癣菌的抗真菌活性。采用冻融法制备脂质体，并用粒度分析仪评估其粒径分布。以羟乙基纤维素（HEC）为凝胶剂制备脂质体凝胶。用Brookfield粘度计测定其流变学特性。
结果表明，精油的最小抑菌体积为0.125 ml，95±0.57%的精油成功包封于脂质体中。GC-MS检测到的精油主要成分有苯酚、1,8-桉叶素、柠檬烯、醇类、蒎烯和松油烯。不同制剂的粒径测定结果显示范围较宽，为40.5至298 nm。所制备的脂质体凝胶流变图中未观察到明显的触变性。
精油的脂质体凝胶制剂可能会提高抗真菌活性。
