氮杂胞苷诱导中国仓鼠卵巢细胞中一个DNA修复基因的重新激活。
Azacytidine-induced reactivation of a DNA repair gene in Chinese hamster ovary cells.
作者信息
Jeggo P A, Holliday R
出版信息
Mol Cell Biol. 1986 Aug;6(8):2944-9. doi: 10.1128/mcb.6.8.2944-2949.1986.
Abstract
Six X-ray-sensitive (xrs) strains of the CHO-K1 cell line were shown to revert at a very high frequency after treatment with 5-azacytidine. This suggested that there was a methylated xrs+ gene in these strains which was structurally intact, but not expressed. The xrs strains did not complement one another, and the locus was autosomally located. In view of the frequency of their isolation and their somewhat different phenotypes, we propose that the xrs strains are mutants derived from an active wild-type gene. However, there is in addition a methylated silent gene present in the genome. Azacytidine treatment reactivated this gene. We present a model for the functional hemizygosity of mammalian cell lines, which is based on the inactivation of genes by de novo hypermethylation. In contrast to results with xrs strains, other repair-defective lines were found not to be reverted by azacytidine.
摘要
CHO-K1细胞系的六个对X射线敏感（xrs）的菌株在用5-氮杂胞苷处理后显示出极高频率的回复突变。这表明在这些菌株中存在一个甲基化的xrs+基因，其结构完整但未表达。xrs菌株之间不能互补，该基因座位于常染色体上。鉴于它们的分离频率和有些不同的表型，我们提出xrs菌株是源自一个活性野生型基因的突变体。然而，基因组中还存在一个甲基化的沉默基因。氮杂胞苷处理使该基因重新激活。我们提出了一个基于基因从头超甲基化导致基因失活的哺乳动物细胞系功能半合子模型。与xrs菌株的结果相反，发现其他修复缺陷型细胞系不会被氮杂胞苷回复突变。
相似文献
1
Azacytidine-induced reactivation of a DNA repair gene in Chinese hamster ovary cells.氮杂胞苷诱导中国仓鼠卵巢细胞中一个DNA修复基因的重新激活。
Mol Cell Biol. 1986 Aug;6(8):2944-9. doi: 10.1128/mcb.6.8.2944-2949.1986.
2
Cytogenetical characterization of Chinese hamster ovary X-ray-sensitive mutant cells xrs 5 and xrs 6. III. Induction of cell killing, chromosomal aberrations and sister-chromatid exchanges by bleomycin, mono- and bi-functional alkylating agents.中国仓鼠卵巢X射线敏感突变细胞xrs 5和xrs 6的细胞遗传学特征。III. 博来霉素、单功能和双功能烷化剂对细胞杀伤、染色体畸变和姐妹染色单体交换的诱导作用
Mutat Res. 1989 Jun;212(2):123-35. doi: 10.1016/0027-5107(89)90063-8.
3
Cytological characterization of Chinese hamster ovary X-ray-sensitive mutant cells, xrs 5 and xrs 6. II. Induction of sister-chromatid exchanges and chromosomal aberrations by X-rays and UV-irradiation and their modulation by inhibitors of poly(ADP-ribose) synthetase and alpha-polymerase.中国仓鼠卵巢X射线敏感突变细胞xrs 5和xrs 6的细胞学特征。II. X射线和紫外线照射诱导的姐妹染色单体交换和染色体畸变及其受聚（ADP - 核糖）合成酶和α - 聚合酶抑制剂的调节
Mutat Res. 1987 Mar;177(1):149-60. doi: 10.1016/0027-5107(87)90030-3.
4
Biochemical and cytogenetical characterization of Chinese hamster ovary X-ray-sensitive mutant cells xrs 5 and xrs 6. V. The correlation of DNA strand breaks and base damage to chromosomal aberrations and sister-chromatid exchanges induced by X-irradiation.中国仓鼠卵巢X射线敏感突变细胞xrs 5和xrs 6的生化及细胞遗传学特征。V. DNA链断裂和碱基损伤与X射线诱导的染色体畸变及姐妹染色单体交换的相关性。
Mutat Res. 1990 Mar;235(2):119-27. doi: 10.1016/0921-8777(90)90065-d.
5
Cytological characterization of Chinese hamster ovary X-ray-sensitive mutant cells xrs 5 and xrs 6. I. Induction of chromosomal aberrations by X-irradiation and its modulation with 3-aminobenzamide and caffeine.中国仓鼠卵巢X射线敏感突变细胞xrs 5和xrs 6的细胞学特征。I. X射线照射诱导染色体畸变及其用3-氨基苯甲酰胺和咖啡因进行调节
Mutat Res. 1987 Mar;177(1):133-48. doi: 10.1016/0027-5107(87)90029-7.
6
Cytogenetical characterization of Chinese hamster ovary X-ray-sensitive mutant cells, xrs 5 and xrs 6. IV. Study of chromosomal aberrations and sister-chromatid exchanges by restriction endonucleases and inhibitors of DNA topoisomerase II.中国仓鼠卵巢X射线敏感突变细胞xrs 5和xrs 6的细胞遗传学特征。IV. 用限制性内切酶和DNA拓扑异构酶II抑制剂研究染色体畸变和姐妹染色单体交换
Mutat Res. 1989 Jun;212(2):137-48. doi: 10.1016/0027-5107(89)90064-x.
7
Biochemical and cytogenetical characterization of Chinese hamster ovary X-ray-sensitive mutant cells xrs 5 and xrs 6. VI. The correlation between UV-induced DNA lesions and chromosomal aberrations, and their modulations with inhibitors of DNA repair synthesis.中国仓鼠卵巢X射线敏感突变细胞xrs 5和xrs 6的生化与细胞遗传学特征。VI.紫外线诱导的DNA损伤与染色体畸变之间的相关性，以及它们受DNA修复合成抑制剂的调节作用。
Mutat Res. 1990 Mar;235(2):129-35. doi: 10.1016/0921-8777(90)90066-e.
8
Use of damaged plasmid to study DNA repair in X-ray sensitive (xrs) strains of Chinese hamster ovary (CHO) cells.使用受损质粒研究中国仓鼠卵巢（CHO）细胞的X射线敏感（xrs）菌株中的DNA修复。
Int J Radiat Biol. 1989 Dec;56(6):951-61. doi: 10.1080/09553008914552411.
9
Sensitivity of Chinese hamster ovary mutants defective in DNA double strand break repair to topoisomerase II inhibitors.DNA双链断裂修复缺陷的中国仓鼠卵巢突变体对拓扑异构酶II抑制剂的敏感性。
Cancer Res. 1989 Dec 15;49(24 Pt 1):7057-63.
10
Repair of DNA single-strand and double-strand breaks in the Chinese hamster xrs 5 mutant cell line as determined by DNA unwinding.通过DNA解旋测定中国仓鼠xrs 5突变细胞系中DNA单链和双链断裂的修复情况。
Mutat Res. 1988 Sep;194(2):93-9. doi: 10.1016/0167-8817(88)90011-9.
引用本文的文献
1
Epigenetic targeting of transposon relics: beating the dead horses of the genome?表观遗传学靶向转座子遗迹：重蹈基因组的覆辙？
Epigenetics. 2022 Nov;17(11):1331-1344. doi: 10.1080/15592294.2021.2022066. Epub 2022 Jan 4.
2
A critical appraisal of clinical epigenetics.临床表观遗传学的批判性评价。
Clin Epigenetics. 2022 Jul 28;14(1):95. doi: 10.1186/s13148-022-01315-6.
3
Restoration of DNA repair mitigates genome instability and increases productivity of Chinese hamster ovary cells.DNA 修复的恢复减轻了基因组不稳定性，并提高了中国仓鼠卵巢细胞的生产力。
Biotechnol Bioeng. 2022 Mar;119(3):963-982. doi: 10.1002/bit.28016. Epub 2022 Jan 5.
4
Epigenetic gene silencing by heterochromatin primes fungal resistance.异染色质介导的表观遗传基因沉默引发真菌抗性。
Nature. 2020 Sep;585(7825):453-458. doi: 10.1038/s41586-020-2706-x. Epub 2020 Sep 9.
5
Single-cell, locus-specific bisulfite sequencing (SLBS) for direct detection of epimutations in DNA methylation patterns.用于直接检测DNA甲基化模式中表观突变的单细胞、位点特异性亚硫酸氢盐测序（SLBS）。
Nucleic Acids Res. 2015 Aug 18;43(14):e93. doi: 10.1093/nar/gkv366. Epub 2015 Apr 19.
6
Sequencing the CHO DXB11 genome reveals regional variations in genomic stability and haploidy.对中国仓鼠卵巢细胞系DXB11进行基因组测序，揭示了基因组稳定性和单倍性的区域差异。
BMC Genomics. 2015 Mar 8;16(1):160. doi: 10.1186/s12864-015-1391-x.
7
Epigenetics and memigenetics.表观遗传学和记忆遗传学。
Cell Mol Life Sci. 2014 Apr;71(7):1117-22. doi: 10.1007/s00018-014-1560-0. Epub 2014 Jan 21.
8
Epimutations mimic genomic mutations of DNMT3A in acute myeloid leukemia.表观遗传突变模拟了急性髓细胞白血病中 DNMT3A 的基因组突变。
Leukemia. 2014 Jun;28(6):1227-34. doi: 10.1038/leu.2013.362. Epub 2013 Nov 27.
9
Mammalian Rad51C contributes to DNA cross-link resistance, sister chromatid cohesion and genomic stability.哺乳动物的Rad51C有助于抵抗DNA交联、姐妹染色单体黏连及基因组稳定性。
Nucleic Acids Res. 2002 May 15;30(10):2172-82. doi: 10.1093/nar/30.10.2172.
10
The C terminus of Ku80 activates the DNA-dependent protein kinase catalytic subunit.Ku80的C末端激活DNA依赖性蛋白激酶催化亚基。
Mol Cell Biol. 1999 May;19(5):3267-77. doi: 10.1128/MCB.19.5.3267.
本文引用的文献
1
Role of methylation in the induced and spontaneous expression of the avian endogenous virus ev-1: DNA structure and gene products.甲基化在禽内源性病毒ev-1诱导性和自发性表达中的作用：DNA结构与基因产物
Mol Cell Biol. 1982 Jun;2(6):638-52. doi: 10.1128/mcb.2.6.638-652.1982.
2
A screening method for isolating DNA repair-deficient mutants of CHO cells.一种用于分离中国仓鼠卵巢细胞DNA修复缺陷型突变体的筛选方法。
Somatic Cell Genet. 1980 May;6(3):391-405. doi: 10.1007/BF01542791.
3
Methylation of deoxycytidine incorporated by excision-repair synthesis of DNA.通过DNA切除修复合成掺入的脱氧胞苷的甲基化。
Cell. 1982 Sep;30(2):509-16. doi: 10.1016/0092-8674(82)90248-3.
4
De novo methylation, expression, and infectivity of retroviral genomes introduced into embryonal carcinoma cells.导入胚胎癌细胞的逆转录病毒基因组的从头甲基化、表达及感染性。
Proc Natl Acad Sci U S A. 1982 Jul;79(13):4098-102. doi: 10.1073/pnas.79.13.4098.
5
Genetic diversity of UV-sensitive DNA repair mutants of Chinese hamster ovary cells.中国仓鼠卵巢细胞紫外线敏感DNA修复突变体的遗传多样性
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3734-7. doi: 10.1073/pnas.78.6.3734.
6
Repair capability and the cellular age response for killing and mutation induction after UV.紫外线照射后的修复能力以及细胞杀伤和突变诱导的细胞年龄反应。
Mutat Res. 1982 Aug;95(2-3):505-14. doi: 10.1016/0027-5107(82)90281-0.
7
DNA methylation decreases in aging but not in immortal cells.DNA甲基化在衰老过程中会减少，但在永生化细胞中不会。
Science. 1983 Jun 3;220(4601):1055-7. doi: 10.1126/science.6844925.
8
Provisional assignment of TPI, GPI, and PEPD to Chinese hamster autosomes 8 and 9: a cytogenetic basis for functional haploidy of an autosomal linkage group in CHO cells.磷酸丙糖异构酶、葡萄糖磷酸异构酶和肽酶D在中华仓鼠常染色体8和9上的临时定位：中国仓鼠卵巢细胞中一个常染色体连锁群功能单倍体的细胞遗传学基础。
Cytogenet Cell Genet. 1983;35(1):15-20. doi: 10.1159/000131830.
9
Inhibition of DNA methylation by chemical carcinogens in vitro.化学致癌物在体外对DNA甲基化的抑制作用。
Cell. 1983 Jan;32(1):239-46. doi: 10.1016/0092-8674(83)90514-7.
10
Ultraviolet radiation-induced metallothionein-I gene activation is associated with extensive DNA demethylation.紫外线辐射诱导的金属硫蛋白-I基因激活与广泛的DNA去甲基化有关。
Cell. 1983 Nov;35(1):207-14. doi: 10.1016/0092-8674(83)90223-4.
Zotero 插件