[变应性鼻炎患者外周血γδT细胞、调节性T细胞及细胞因子白细胞介素-17和转化生长因子-β1的表达]

[Expression of peripheral blood gammadelta T cells, treg cells and cytokines IL-17 and TGF-beta1 in patients with allergic rhinitis].

作者信息

Huang Xue-Kun, Yang Qin-Tai, Chen Yu-Lian, Zhang Fu-Cheng, Zhang Ge-Hua

机构信息

Department of Otorhinolaryngology, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

出版信息

Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2013 Jul;48(7):544-8.

PMID:24313201

Abstract

OBJECTIVE

To investigate the expression of peripheral blood gammadelta T cells/CD4 CD25+ regulatory T cells(Treg) and cytokines interleukin 17 (IL-17) and transforming growth factor beta1 (TGF-beta1) in patients with allergic rhinitis.

METHODS

From March 2012 to July 2012, 32 patients with allergic rhinitis (AR group) and 20 healthy control subjects (control group) were collected. The expression of peripheral blood gammadelta T cells/Treg cells were measured by flow cytometry and the levels of IL-17 and TGF-beta1 were evaluated by ELISA. SPSS 16.0 software was used to analyze the data.

RESULTS

The percentages of gammadeltaT cells in AR group were (13.30 +/- 8.62)%, which was significantly higher (t = 5.18, P < 0.01) than those in control group (5.18 +/- 1.86)%. The percentages of Treg cells in AR group were (1.75 +/- 0.56)%, which were significantly lower (t = 7.46, P < 0. 01) than those in control group (4.76 +/- 1.74)%. The IL-17 levels in AR group were (668.55 +/- 45.15) pg/ml, which were also significantly higher (t = 8.97, P < 0.01) than those in control group (573.53 +/- 17.42) pg/ml. The TGF-beta1 levels in AR group were (0.34 +/- 0.04) pg/ml, which were also significantly lower (t = 9.51, P < 0.01) than those in control group (0.49 +/- 0.06) pg/ml. There was a negative correlation between the percentages of gammadelta T cells and Treg cells (r = -0.561, P < 0.01). There was a negative correlation between the percentages of gammadelta T cells and TGF-beta1 levels (r = -0.622, P < 0.01). A positive correlation was shown between the percentages of gammadelta T cells and IL-17 levels in AR (r = 0.469, P < 0.01). A positive correlation was shown between the percentages of Treg cells and TGF-beta1 levels in AR (r = 0.738, P < 0.01). There was no correlation between IL-17 levels and the percentages of Treg cells or TGF-beta1 levels (r value was -0.111, -0.196, all P > 0.05).

CONCLUSION

There are imbalances of gammadelta T and Treg cells in peripheral blood of patients with allergic rhinitis. gammadelta T cells may be the main cell to produce IL-17, which may play an important role in allergic rhinitis.

摘要

目的

探讨变应性鼻炎患者外周血γδT细胞/CD4 CD25+调节性T细胞（Treg）及细胞因子白细胞介素17（IL-17）和转化生长因子β1（TGF-β1）的表达情况。

方法

收集2012年3月至2012年7月期间32例变应性鼻炎患者（AR组）及20例健康对照者（对照组）。采用流式细胞术检测外周血γδT细胞/Treg细胞的表达，酶联免疫吸附测定法（ELISA）评估IL-17和TGF-β1水平。应用SPSS 16.0软件进行数据分析。

结果

AR组γδT细胞百分比为（13.30±8.62）%，显著高于对照组（5.18±1.86）%（t = 5.18，P < 0.01）。AR组Treg细胞百分比为（1.75±0.56）%，显著低于对照组（4.76±1.74）%（t = 7.46，P < 0.01）。AR组IL-17水平为（668.55±45.15）pg/ml，也显著高于对照组（573.53±17.42）pg/ml（t = 8.97，P < 0.01）。AR组TGF-β1水平为（0.34±0.04）pg/ml，也显著低于对照组（0.49±0.06）pg/ml（t = 9.51，P < 0.01）。γδT细胞百分比与Treg细胞百分比呈负相关（r = -0.561，P < 0.01）。γδT细胞百分比与TGF-β1水平呈负相关（r = -0.622，P < 0.01）。AR组γδT细胞百分比与IL-17水平呈正相关（r = 0.469，P < 0.01）。AR组Treg细胞百分比与TGF-β1水平呈正相关（r = 0.738，P < 0.01）。IL-17水平与Treg细胞百分比或TGF-β1水平无相关性（r值分别为-0.111、-0.196，均P > 0.05）。