Xu F F, Yu S Q, Zhao C L, Che N, Jin L, Wang Q L, Ge R M
Department of Otorhinolaryngology Head and Neck Surgery, Tongji Hospital, Tongji University, Shanghai 200065, China.
Department of Otorhinolaryngology Head and Neck Surgery, Shanghai Tenth People's Hospital, Shanghai 200072, China.
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2017 Jul 7;52(7):506-511. doi: 10.3760/cma.j.issn.1673-0860.2017.07.006.
To explore the effect of hydrogen-rich saline on the CD4(+) CD25(+) Foxp3(+) Treg cells in a guinea pig model of allergic rhinitis (AR) and investigate the underling anti-inflammatory mechanism. Using random number table, eighteen guinea pigs were divided into three groups (control group/AR group/HRS group, =6 of each group). AR guinea pig model was built with ovalbumin and aluminum. The guinea pigs were injected with hydrogen-rich saline (HRS group) for ten days after sensitation. And control group was injected with equal normal saline at the same time. Number of sneezes, degree of runny nose and nasal rubbing movements were scored. Peripheral blood eosinophil count was recorded. The content of interleukin 10(IL-10) and transforming growth factor β (TGF-β) in the serum were detected by enzyme-linked immunosorbent assay (ELISA). Immunohistochemical method was taken to detect IL-10 and TGF-β in nasal mucosa. The proportion of CD4(+) CD25(+) Foxp3(+) T cells in the CD4(+) T cells of spleen and peripheral blood were determined with flow cytometry. SPSS 17.0 software was used to analyze the data. There was significant difference in symptom scores among them. The scores of AR group preceded control group, and HRS could decrease the scores of AR ((6.29±1.79) (1.01±0.71), (4.50±0.84) (6.29±1.79), =24.725, all <0.05). The highest number of eosinophils in the peripheral blood belonged to control group, and the number of eosinophils were dramatically reduced after HRS administration ((0.41±0.05)×10(9)/L (0.25±0.03 )×10(9)/L, (0.32±0.03)×10(9)/L (0.41±0.05)×10(9)/L, =70.05, all <0.05). The content of IL-10 and TGF-β in control group is peak ((86.88±17.17) pg/ml, (598.28±72.70) pg/ml, respectively), and compared with AR group, HRS also increased the expression of IL-10 and TGF-β of peripheral blood ((72.54±11.75) pg/ml (53.49±10.07) pg/ml, (530.23±57.15) pg/ml (482.69±65.96) pg/ml, value was 28.357, 14.128, respectively, all <0.05). The proportion of CD4(+) CD25(+) Foxp3(+) Treg cells in controls exceeded HRS group and AR group (1.81%±0.10%, 1.29%±0.74%, respectively), and HRS treatment increased the ratio of CD4(+) CD25(+) Foxp3(+) Treg cells than AR group of peripheral blood ((1.50%±0.11%) (1.15%±0.11%), =168.96, <0.05). But there was no significant diferences in splene tissue ((1.01%±0.08%) (0.98%±0.09%), =97.381, >0.05). Both the number and the cytokine secretion of CD4(+) CD25(+) Foxp3(+) Treg cells are decreased in AR group, HRS may inhibit inflammatory response and ameliorate AR via improving the number and the cytokine secretion.
探讨富氢盐水对变应性鼻炎（AR）豚鼠模型中CD4(+)CD25(+)Foxp3(+)调节性T细胞（Treg细胞）的影响，并研究其潜在的抗炎机制。采用随机数字表法，将18只豚鼠分为3组（对照组/AR组/富氢盐水组，每组6只）。用卵清蛋白和氢氧化铝建立AR豚鼠模型。致敏后，对豚鼠注射富氢盐水（富氢盐水组），持续10天。同时，对对照组注射等量的生理盐水。记录喷嚏次数、流涕程度和鼻摩擦动作，并进行评分。记录外周血嗜酸性粒细胞计数。采用酶联免疫吸附测定（ELISA）法检测血清中白细胞介素10（IL-10）和转化生长因子β（TGF-β）的含量。采用免疫组织化学方法检测鼻黏膜中IL-10和TGF-β。用流式细胞术测定脾脏和外周血CD4(+)T细胞中CD4(+)CD25(+)Foxp3(+)T细胞的比例。采用SPSS 17.0软件进行数据分析。各组间症状评分差异有统计学意义。AR组评分高于对照组，富氢盐水可降低AR组评分（(6.29±1.79) (1.01±0.71)，(4.50±0.84) (6.29±1.79)，=24.725，均<0.05）。外周血中嗜酸性粒细胞数量最多的是对照组，富氢盐水给药后嗜酸性粒细胞数量显著减少（(0.41±0.05)×10(9)/L (0.25±0.03 )×10(9)/L，(0.32±0.0
