Department of Agricultural Sciences, University of Bologna , Viale G. Fanin 44, 40127 Bologna, Italy.
J Agric Food Chem. 2014 Jan 15;62(2):337-47. doi: 10.1021/jf403436t. Epub 2013 Dec 30.
Ralstonia solanacearum (Rs) and Clavibacter michiganensis subsp. sepedonicus (Cms) are the bacterial causal agents of potato brown and ring rot, respectively, and are included in the A2 list of quarantine pathogens in Europe. Identification by GC-MS analysis of volatile organic compounds from Rs or Cms cultured on different nutrient media was performed. GC-MS and PTR-MS analysis were carried out also on unwounded potato tubers infected with the same pathogens. Infected tubers were produced by experimental inoculations of the plants. In in vitro experiments, Rs or Cms emitted volatile compounds, part of which were specific disease markers of potato (2-propanol and 3-methylbutanoic acid), mainly originating from bacterial metabolism (i.e., amino acid degradation, carbohydrate and fatty acid oxidation). In potato tubers, pathogen metabolism modified the volatile compound pattern emitted from healthy samples. Both bacteria seem to accelerate metabolic processes ongoing in potatoes and, in the case of Rs, disease markers (1-hepten-3-ol, 3,6-dimethyl-3-octanone, 3-ethyl-3-methylpentane, 1-chloroctane, and benzothiazole) were identified.
青枯雷尔氏菌（Rs）和茄科劳尔氏菌密执安亚种（Cms）分别是引起马铃薯褐腐病和环腐病的细菌病原物，被列入欧洲检疫性有害生物 A2 名单。本研究通过对 Rs 和 Cms 在不同营养培养基上培养所产生的挥发性有机化合物进行气相色谱-质谱（GC-MS）分析来进行鉴定。还对感染相同病原菌但未受伤的马铃薯块茎进行了 GC-MS 和质子转移反应-质谱（PTR-MS）分析。受感染的块茎是通过对植物进行实验接种产生的。在体外实验中，Rs 或 Cms 会释放挥发性化合物，其中部分是马铃薯的特定疾病标志物（2-丙醇和 3-甲基丁酸），主要源自细菌代谢（即，氨基酸降解、碳水化合物和脂肪酸氧化）。在马铃薯块茎中，病原菌代谢改变了健康样本中释放的挥发性化合物模式。这两种细菌似乎都加速了马铃薯中正在进行的代谢过程，并且在 Rs 的情况下，还鉴定出了疾病标志物（1-庚烯-3-醇、3,6-二甲基-3-辛酮、3-乙基-3-甲基戊烷、1-氯辛烷和苯并噻唑）。
