Department of Environmental Biotechnology, Lodz University of Technology, Wólczańska 171/173, 90-530 Łódź, Poland.
Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Wólczańska 171/173, 90-530 Łódź, Poland.
Molecules. 2022 Jun 9;27(12):3708. doi: 10.3390/molecules27123708.
The feasibility of early disease detection in potato seeds storage monitoring of volatile organic compounds (VOCs) and plant physiological markers was evaluated using 10 fungal and bacterial pathogens of potato in laboratory-scale experiments. Data analysis of HS-SPME-GC-MS revealed 130 compounds released from infected potatoes, including sesquiterpenes, dimethyl disulfide, 1,2,4-trimethylbenzene, 2,6,11-trimethyldodecane, benzothiazole, 3-octanol, and 2-butanol, which may have been associated with the activity of , and . In turn, acetic acid was detected in all infected samples. The criteria of selection for volatiles for possible use as incipient disease indicators were discussed in terms of potato physiology. The established physiological markers proved to demonstrate a negative effect of phytopathogens infecting seed potatoes not only on the kinetics of stem and root growth and the development of the entire root system, but also on gas exchange, chlorophyll content in leaves, and yield. The negative effect of phytopathogens on plant growth was dependent on the time of planting after infection. The research also showed different usefulness of VOCs and physiological markers as the indicators of the toxic effect of inoculated phytopathogens at different stages of plant development and their individual organs.
采用实验室规模实验，评估了使用 10 种马铃薯真菌和细菌病原体对马铃薯种子储存中挥发性有机化合物（VOCs）和植物生理标记物进行早期疾病检测的可行性。HS-SPME-GC-MS 数据分析显示，从感染的马铃薯中释放出 130 种化合物，包括倍半萜烯、二甲基二硫醚、1,2,4-三甲基苯、2,6,11-三甲基十二烷、苯并噻唑、3-辛醇和 2-丁醇，这些化合物可能与 的活性有关。反过来，所有感染的样本中都检测到了乙酸。根据马铃薯生理学，讨论了将挥发性物质作为早期疾病指标的选择标准。所建立的生理标记物被证明不仅对茎和根生长的动力学以及整个根系的发育，而且对气体交换、叶片叶绿素含量和产量产生负面影响。病原体对植物生长的负面影响取决于感染后种植的时间。该研究还表明，在植物发育的不同阶段及其各个器官中，VOCs 和生理标记物作为接种植物病原体毒性作用的指标具有不同的用途。
