Donaldson J, Dangarembizi R, Mtetwa B, Madziva M T, Erlwanger K H
Faculty of Health Sciences, School of Physiology, University of the Witwatersrand, Johannesburg, South Africa.
J Anim Physiol Anim Nutr (Berl). 2014 Oct;98(5):867-74. doi: 10.1111/jpn.12149. Epub 2013 Dec 9.
To investigate the progressive effects of a high-fat diet on erythrocyte osmotic fragility, growth performance and serum lipid concentrations in Guinea fowl and Muscovy ducks, 36 Guinea fowl and 36 Muscovy ducks were divided into two groups, for each species, and fed either a standard (STD = commercial poultry feed) or high-fat diet (HFD = commercial poultry feed with 20% palm oil and 2% lard) for up to 12 weeks. After 4, 8 and 12 weeks on the diets, six birds from each group were euthanized and blood samples collected. Osmotic fragility was assessed by measuring the haemoglobin released by erythrocytes placed in serially diluted solutions of phosphate-buffered saline, spectrophotometrically. Serum triglyceride and cholesterol concentrations were also determined. Fragiligrams from erythrocytes from both species of birds on the HFD were not different to those on the STD. However, Muscovy duck erythrocytes were more resistant to haemolysis compared with Guinea fowl erythrocytes. Final body mass and serum triglyceride levels were not significantly different (p > 0.05, anova) between the birds in the HFD and STD groups, for both species of birds. In contrast, serum cholesterol levels were significantly higher in birds on the HFD compared with those on the STD, after 4, 8 and 12 weeks of feeding, for both species of birds. Feeding Guinea fowl and Muscovy ducks a high-fat diet for up to 12 weeks resulted in hypercholesterolaemia but had no effect on final body mass, erythrocyte osmotic fragility or serum triglyceride concentrations in either bird species.
为研究高脂饮食对珍珠鸡和番鸭红细胞渗透脆性、生长性能及血脂浓度的渐进性影响，将36只珍珠鸡和36只番鸭各分为两组，分别饲喂标准日粮（STD = 商用家禽饲料）或高脂日粮（HFD = 含20%棕榈油和2%猪油的商用家禽饲料），持续12周。在日粮喂养4、8和12周后，每组处死6只鸟并采集血样。通过分光光度法测量置于系列稀释磷酸盐缓冲盐溶液中的红细胞释放的血红蛋白来评估渗透脆性。同时测定血清甘油三酯和胆固醇浓度。高脂日粮组两种鸟类红细胞的脆性图与标准日粮组无差异。然而，与珍珠鸡红细胞相比，番鸭红细胞对溶血更具抵抗力。两种鸟类高脂日粮组和标准日粮组的最终体重和血清甘油三酯水平无显著差异（p>0.05，方差分析）。相比之下，在喂养4、8和12周后，两种鸟类高脂日粮组的血清胆固醇水平均显著高于标准日粮组。给珍珠鸡和番鸭饲喂高脂日粮长达12周会导致高胆固醇血症，但对两种鸟类的最终体重、红细胞渗透脆性或血清甘油三酯浓度均无影响。
