Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia (UPM), Serdang, Malaysia.
J Appl Microbiol. 2014 Apr;116(4):903-10. doi: 10.1111/jam.12410. Epub 2013 Dec 20.
To isolate a bacterial strain capable of biotransforming ferulic acid, a major component of lignin, into vanillin and vanillic acid by a rapid colorimetric screening method.
For the production of vanillin, a natural aroma compound, we attempted to isolate a potential strain using a simple screening method based on pH change resulting from the degradation of ferulic acid. The strain Pseudomonas sp. AZ10 UPM exhibited a significant result because of colour changes observed on the assay plate on day 1 with a high intensity of yellow colour. The biotransformation of ferulic acid into vanillic acid by the AZ10 strain provided the yield (Yp/s ) and productivity (Pr ) of 1·08 mg mg(-1) and 53·1 mg L(-1) h(-1) , respectively. In fact, new investigations regarding lignin degradation revealed that the strain was not able to produce vanillin and vanillic acid directly from lignin; however, partially digested lignin by mixed enzymatic treatment allowed the strain to produce 30·7 mg l(-1) and 1·94 mg l(-1) of vanillic acid and biovanillin, respectively.
(i) The rapid colorimetric screening method allowed the isolation of a biovanillin producer using ferulic acid as the sole carbon source. (ii) Enzymatic treatment partially digested lignin, which could then be utilized by the strain to produce biovanillin and vanillic acid.
To the best of our knowledge, this is the first study reporting the use of a rapid colorimetric screening method for bacterial strains producing vanillin and vanillic acid from ferulic acid.
采用快速比色筛选法，从木质素的主要成分阿魏酸中分离出能将其转化为香草醛和香草酸的细菌菌株。
为了生产香草醛这种天然香料化合物，我们尝试采用一种基于阿魏酸降解导致 pH 值变化的简单筛选方法来分离潜在的菌株。假单胞菌 AZ10 UPM 菌株表现出显著的结果，因为在第 1 天的测定板上观察到颜色变化，颜色强度为黄色。AZ10 菌株将阿魏酸转化为香草酸的生物转化提供了 1.08 mg mg(-1)的产率 (Yp/s) 和 53.1 mg L(-1) h(-1)的生产力 (Pr)。事实上，关于木质素降解的新研究表明，该菌株不能直接从木质素产生香草醛和香草酸；然而，通过混合酶处理对木质素进行部分消化，允许该菌株分别产生 30.7 mg l(-1)和 1.94 mg l(-1)的香草酸和生物香草醛。
(i) 快速比色筛选法允许使用阿魏酸作为唯一碳源分离生物香草醛产生菌。(ii) 酶处理部分消化木质素，然后该菌株可以利用木质素来生产生物香草醛和香草酸。
据我们所知，这是首次报道使用快速比色筛选法从阿魏酸中分离生产香草醛和香草酸的细菌菌株。
