Department of Biology, Faculty of Sciences, University of Isfahan, Azadi Sq., Daneshgah St., Isfahan 81746-73441, Iran.
N Biotechnol. 2011 Oct;28(6):656-64. doi: 10.1016/j.nbt.2011.06.009. Epub 2011 Jun 13.
In this study a novel strain was isolated with the capability to grow on eugenol as a source of carbon and energy. This strain was identified as Pseudomonas resinovorans (GenBank accession no. HQ198585) based on phenotypic characterization and phylogenetic analysis of 16S rDNA gene. The intermediates coniferyl alcohol, coniferyl aldehyde, ferulic acid, vanillin and vanillic acid were detected in the culture supernatant during eugenol biotransformation with this strain. The products were confirmed by thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and spectral data achieved from UV-vis, FTIR and mass spectroscopy. Using eugenol as substrate and resting cells of P. resinovorans SPR1, which were harvested at the end of the exponential growth phase, without further optimization 0.24 g/L vanillin (molar yield of 10%) and 1.1g/L vanillic acid (molar yield of 44%) were produced after 30 h and 60 h biotransformation, respectively. The current work gives the first evidence for the eugenol biotransformation by P. resinovorans.
在这项研究中，分离到了一株能够利用丁香酚作为碳源和能源生长的新型菌株。根据表型特征和 16S rDNA 基因的系统发育分析，该菌株被鉴定为假单胞菌（GenBank 登录号 HQ198585）。在丁香酚生物转化过程中，该菌株的培养上清液中检测到中间产物松柏醇、松柏醛、阿魏酸、香草醛和香草酸。通过薄层层析（TLC）、高效液相色谱（HPLC）和从紫外可见光谱、傅里叶变换红外光谱和质谱获得的光谱数据对产物进行了确认。以丁香酚为底物，利用在指数生长末期收获的假单胞菌 SPR1 静止细胞，在没有进一步优化的情况下，30 h 和 60 h 生物转化后分别产生了 0.24 g/L 香草醛（摩尔收率为 10%）和 1.1 g/L 香草酸（摩尔收率为 44%）。本研究首次证明了假单胞菌对丁香酚的生物转化。
