Andreasen R B, Biberfeld P, Ost A, Reizenstein P, Olsson L
Scand J Haematol. 1986 Oct;37(4):323-32. doi: 10.1111/j.1600-0609.1986.tb02321.x.
The immunological phenotypes of leukemia cell samples from 60 patients, of whom 54 had acute myeloid leukemia (AML), were assessed with a panel of monoclonal antibodies (Mabs) with specificity for the following epitopes: Epitopes associated with myeloid leukemia cells, Epitopes expressed only on immature myeloid cells (or subsets) and on monocytes, Epitopes only expressed on granulocytes or on granulocytes and mature myeloid cells (promyelocytes, myelocytes and monocytes), Epitopes on HLA-class II (DR) and HLA-class I molecules and on insulin receptors. This panel of Mabs proved useful to identify leukemia cells in blood and to assess their myeloid origin. The panel of Mabs was found also to be useful for immunophenotyping of leukemia cells. Furthermore, the analysis revealed considerable variations in the immunological phenotype of AML cells, reflecting antigenic heterogeneity within the individual leukemia cell population as well as abnormal or no expression of histocompatibility antigens and insulin receptors in some samples. Some of the Mabs bound preferentially to subgroups in the French-American-British (FAB) classification.
对60例患者的白血病细胞样本的免疫表型进行了评估，其中54例患有急性髓系白血病（AML），使用一组对以下表位具有特异性的单克隆抗体（Mab）：与髓系白血病细胞相关的表位、仅在未成熟髓系细胞（或亚群）和单核细胞上表达的表位、仅在粒细胞或粒细胞和成熟髓系细胞（早幼粒细胞、中幼粒细胞和单核细胞）上表达的表位、HLA - II类（DR）和HLA - I类分子以及胰岛素受体上的表位。这组Mab被证明有助于识别血液中的白血病细胞并评估其髓系起源。这组Mab还被发现可用于白血病细胞的免疫表型分析。此外，分析显示AML细胞的免疫表型存在相当大的差异，反映了单个白血病细胞群体内的抗原异质性以及某些样本中组织相容性抗原和胰岛素受体的异常表达或无表达。一些Mab优先与法国 - 美国 - 英国（FAB）分类中的亚组结合。
