Bloom J W, Mitra G
Thromb Res. 1986 Oct 15;44(2):207-15. doi: 10.1016/0049-3848(86)90136-2.
The interactions of human blood clotting Factor VIII:c with 2 monoclonal antibodies, C7F7 and BD10, were examined with an enzyme-linked immunosorbent assay (ELISA) method. A Scatchard-Sips plot approach to data analysis was used to calculate an average affinity (Ko), valence (n), and heterogeneity index (a) for each interaction. Using a Factor VIII:c coating ELISA method, the following constants were determined: BD10: Ko = 5.48 X 10(8) M-1; n = 0.31; a = 0.97; C7F7: Ko = 2.3 X 10(11) M-1; n = 0.018; a = 0.77.
采用酶联免疫吸附测定（ELISA）方法检测人凝血因子VIII:c与两种单克隆抗体C7F7和BD10的相互作用。运用Scatchard-Sips数据分析方法计算每次相互作用的平均亲和力（Ko）、价数（n）和异质性指数（a）。采用因子VIII:c包被ELISA方法测定了以下常数：BD10：Ko = 5.48×10⁸ M⁻¹；n = 0.31；a = 0.97；C7F7：Ko = 2.3×10¹¹ M⁻¹；n = 0.018；a = 0.77。
