剪接体古老核心中的 snRNA 催化剂。

snRNA catalysts in the spliceosome's ancient core.

机构信息

Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.

出版信息

Cell. 2013 Dec 5;155(6):1213-5. doi: 10.1016/j.cell.2013.11.022.

DOI:10.1016/j.cell.2013.11.022

PMID:24315092

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4162089/

Abstract

The spliceosome, an assembly of snRNAs and proteins, catalyzes the removal of introns from premessenger RNAs. A new study identifies specific phosphates in the U2-U6 snRNA complex that position two catalytic metals. Remarkably, these correspond precisely to metal-binding phosphates in a homologous structure of Group II self-splicing introns, long proposed to be the ribozyme progenitor of spliceosome.

摘要

剪接体是由 snRNA 和蛋白质组成的复合物，可催化前体 mRNA 中内含子的切除。一项新的研究鉴定了 U2-U6 snRNA 复合物中的特定磷酸基团，这些磷酸基团可定位两个催化金属。值得注意的是，这些磷酸基团与 II 组自我剪接内含子的同源结构中的金属结合磷酸基团完全对应，而 II 组自我剪接内含子长期以来被认为是剪接体核酶的前体。