来自携带DQw3单倍型的单个DQ分子上的多个表位。

Multiple epitopes on a single DQ molecule from the DQw3-carrying haplotypes.

作者信息

Maeda H, Hirata R, Kambayashi H, Koning F, Schreuder G M

出版信息

Tissue Antigens. 1986 Sep;28(3):136-45. doi: 10.1111/j.1399-0039.1986.tb00473.x.

DOI:10.1111/j.1399-0039.1986.tb00473.x

Abstract

Multiple polymorphisms on the DQ molecule(s) have been detected by monoclonal antibodies (MoAbs). Among these, TA10 and IIB3 have been described as two new alleles in the DQ region other than the conventional DQw1-w3 allelism. The TA10 specificity is DQw3-related and is in linkage disequilibrium with DR5 and weakly associated with DR4. The IIB3 specificity is DQw1-related and is in linkage disequilibrium with DR2, DR4, DRw9 and DRw13. Thus, the DQw3-carrying haplotypes are either positive with TA10 or IIB3. The molecular and topological analysis has revealed that both TA10 and IIB3 determinants were expressed on a single DQ molecule that also carried the DQw3 determinants on DR5 and DR4 cell lines, respectively. Thus, a single DQ molecule generated multiple epitopes detected by alloantisera and/or MoAbs at least on the DQw3-carrying haplotypes. These would be useful for unraveling the largely unknown functions of the DQ class II molecules.

摘要

通过单克隆抗体（MoAbs）已检测到DQ分子上的多个多态性。其中，TA10和IIB3已被描述为DQ区域中除传统的DQw1 - w3等位基因之外的两个新等位基因。TA10特异性与DQw3相关，与DR5处于连锁不平衡状态，与DR4弱相关。IIB3特异性与DQw1相关，与DR2、DR4、DRw9和DRw13处于连锁不平衡状态。因此，携带DQw3的单倍型要么TA10呈阳性，要么IIB3呈阳性。分子和拓扑分析表明，TA10和IIB3决定簇均在单个DQ分子上表达，该分子在DR5和DR4细胞系上分别还携带DQw3决定簇。因此，至少在携带DQw3的单倍型上，单个DQ分子产生了多个可被同种抗血清和/或单克隆抗体检测到的表位。这些对于揭示II类DQ分子在很大程度上未知的功能将是有用的。