Department of Pharmacology and Toxicology, University of Ghana School of Pharmacy, Ghana.
Department of Physiology, University of Ghana Medical School, College of Health Sciences, P.O. BOX 4236, Ghana.
J Ethnopharmacol. 2014 Feb 3;151(2):891-6. doi: 10.1016/j.jep.2013.11.050. Epub 2013 Dec 4.
Kalanchoe intergra (Ki) leaf extract is an orally administered multipurpose plant medicine in Ghana and other parts of the world for the treatment of ulcers, pain and adenoma of the prostate gland. There is paucity of information concerning its short-term usage. The present study is aimed at conducting histopathological and biochemical studies in a 14-day sub-acute toxicity studies using female Sprague-Dawley rats.
Crude extract of Ki leaves was prepared and freeze-dried. A 14-day sub-acute toxicity studies was conducted using 2 week old nulliparous and non-pregnant female Sprague-Dawley rats (120-150g). Reconstituted Ki was administered at a dosage of 900mgkg(-1) (high dose), 300mgkg(-1) with a control group receiving an equivalent volume of distilled water (as vehicle) by gastric lavage. Histopathological studies of major organs and blood chemistry analysis were performed on blood obtained via cardiac puncture into EDTA tubes after euthanisation.
There was a significant decrease in urea (p<0.016) and creatinine levels (p<0.001) in both the high and low dose groups. There was an increase in ALP levels (P=0.01) in both the high and low dose groups. ALT and AST rather decreased significantly in both the high and low dose groups (p<0.0001). Histopathological results did not show any abnormalities in all the H&E stained paraffin sections. Thus the photomicrographs of the liver, kidney and heart were within histopathological limits.
Ki leaf extract is non-toxic when administered by the oral route over a time period of 14 days at the above doses.
在加纳和世界其他地区，马齿苋（Ki）叶提取物是一种口服多用途植物药物，用于治疗溃疡、疼痛和前列腺腺瘤。关于其短期使用的信息很少。本研究旨在使用雌性 Sprague-Dawley 大鼠进行为期 14 天的亚急性毒性研究中的组织病理学和生化研究。
制备并冻干 Ki 叶的粗提取物。使用 2 周龄未产和未怀孕的雌性 Sprague-Dawley 大鼠（120-150g）进行为期 14 天的亚急性毒性研究。将复溶的 Ki 以 900mgkg(-1)（高剂量）和 300mgkg(-1)的剂量给药，对照组给予等体积的蒸馏水（作为载体）通过灌胃给药。安乐死后通过心脏穿刺到 EDTA 管中获得血液，进行主要器官的组织病理学研究和血液化学分析。
高剂量和低剂量组的尿素（p<0.016）和肌酐水平（p<0.001）均显著降低。高剂量和低剂量组的 ALP 水平均升高（P=0.01）。高剂量和低剂量组的 ALT 和 AST 均显著降低（p<0.0001）。所有 H&E 染色石蜡切片的组织病理学结果均未显示任何异常。因此，肝脏、肾脏和心脏的光镜照片均在组织病理学范围内。
Ki 叶提取物在上述剂量下经口服给药 14 天是无毒的。
