Department of Biosystems and Agricultural Engineering, Oklahoma State University, Stillwater, OK 74078, USA.
Department of Biosystems and Agricultural Engineering, Oklahoma State University, Stillwater, OK 74078, USA.
Bioresour Technol. 2014;152:337-46. doi: 10.1016/j.biortech.2013.11.015. Epub 2013 Nov 14.
Higher alcohols such as n-butanol and n-hexanol have higher energy density than ethanol, are more compatible with current fuel infrastructure, and can be upgraded to jet and diesel fuels. Several organisms are known to convert syngas to ethanol, but very few can produce higher alcohols alone. As a potential solution, mixed culture fermentation between the syngas fermenting Alkalibaculum bacchi strain CP15 and propionic acid producer Clostridium propionicum was studied. The monoculture of CP15 produced only ethanol from syngas without initial addition of organic acids to the fermentation medium. However, the mixed culture produced ethanol, n-propanol and n-butanol from syngas. The addition of propionic acid, butyric acid and hexanoic acid to the mixed culture resulted in a 50% higher conversion efficiency of these acids to their respective alcohols compared to CP15 monoculture. These findings illustrate the great potential of mixed culture syngas fermentation in production of higher alcohols.
高级醇如正丁醇和正己醇的能量密度高于乙醇，与现有燃料基础设施更兼容，并且可以升级为喷气燃料和柴油燃料。已知有几种生物可以将合成气转化为乙醇，但很少有生物可以单独生产高级醇。作为一种潜在的解决方案，研究了将合成气发酵的 Alkalibaculum bacchi CP15 菌株和丙酸生产者 Clostridium propionicum 之间的混合培养发酵。在没有向发酵培养基中初始添加有机酸的情况下，CP15 单培养物仅从合成气中产生乙醇。然而，混合培养物可以从合成气中产生乙醇、正丙醇和正丁醇。与 CP15 单培养物相比，向混合培养物中添加丙酸、丁酸和己酸可使这些酸转化为各自的醇的转化率提高 50%。这些发现说明了混合培养物合成气发酵在生产高级醇方面的巨大潜力。
