Department of Biosystems Engineering, Auburn University, Auburn, AL 36849, USA.
Department of Biosystems Engineering, Auburn University, Auburn, AL 36849, USA; Center for Bioenergy and Bioproducts, Auburn University, Auburn, AL 36849, USA.
Bioresour Technol. 2016 Oct;218:1055-63. doi: 10.1016/j.biortech.2016.07.071. Epub 2016 Jul 21.
Clostridium carboxidivorans P7 is a strict anaerobic bacterium capable of converting syngas to biofuels. However, its fermentation profiles is poorly understood. Here, various end-products, including acetic acid, butyric acid, hexanoic acid, ethanol and butanol were supplemented to evaluate their effects on fermentation profiles in C. carboxidivorans at two temperatures. At 37°C, fatty acids addition likely led to more corresponding alcohols production. At 25°C, C2 and C4 fatty acids supplementation resulted in more corresponding higher fatty acids, while supplemented hexanoic acid increased yields of C2 and C4 fatty acids and hexanol. Supplementation of ethanol or butanol caused increased production of C2 and C4 acids at both temperatures; however, long-chain alcohols were still more likely produced at lower temperature. In conclusion, fermentation profiles of C. carboxidivorans can be changed in respond to pre-added end-products and carbon flow may be redirected to desired products by controlling culture conditions.
羧化梭菌 P7 是一种严格的厌氧细菌，能够将合成气转化为生物燃料。然而，其发酵谱仍不清楚。在这里，我们添加了各种终产物，包括乙酸、丁酸、己酸、乙醇和丁醇，以评估它们在两种温度下对羧化梭菌发酵谱的影响。在 37°C 时，添加脂肪酸可能导致更多相应的醇类生成。在 25°C 时，添加 C2 和 C4 脂肪酸导致更多相应的更高脂肪酸生成，而添加己酸增加了 C2 和 C4 脂肪酸和己醇的产量。在两种温度下，乙醇或丁醇的添加都会导致 C2 和 C4 酸产量增加；然而，在较低温度下，仍然更有可能产生长链醇。总之，羧化梭菌的发酵谱可以响应预先添加的终产物而改变，并且通过控制培养条件，碳流可能会被重新引导到所需的产物上。
