Mrázková O, Grim M, Carlson B M
Am J Anat. 1986 Oct;177(2):141-8. doi: 10.1002/aja.1001770203.
This study of the capillaries in rat skeletal muscle involved the use of a histochemical method that allows one to distinguish between arterial and venous portions of capillaries. Under controlled staining conditions, the arterial portion of the capillary bed reacts positively for alkaline phosphatase (AP) activity, and the venous portion is positive for dipeptidyl peptidase IV (DPP IV) activity. A short transitional capillary segment is positive for the activity of both enzymes. Capillaries of the normal soleus muscle and the red and white portions of the sternomastoid muscle have been quantitatively analyzed. Quantitative data demonstrated differences in capillary dimensions among the muscles studied. Capillaries of the white part of the sternomastoid were the longest, and they had the shortest DPP IV-positive segment (8% of the total capillary length). Capillaries of the soleus muscle were the shortest, and they also had short DPP IV-positive segments (16%). In contrast, the DPP IV-positive segments of the red part of the sternomastoid occupied 60% of the total capillary length. Survey cross sections reveal a mosaic distribution of patches of capillaries stained for AP and DPP IV activity. This study reveals that within given bundles of muscle fibers, the capillaries that run parallel to the muscle fibers are aligned relative to one another in such a manner that their arterial and venous segments are in register.
这项对大鼠骨骼肌毛细血管的研究采用了一种组织化学方法，该方法能让人区分毛细血管的动脉部分和静脉部分。在可控的染色条件下，毛细血管床的动脉部分碱性磷酸酶（AP）活性呈阳性反应，静脉部分二肽基肽酶IV（DPP IV）活性呈阳性。一个短的过渡性毛细血管段两种酶的活性均呈阳性。对正常比目鱼肌以及胸锁乳突肌的红色和白色部分的毛细血管进行了定量分析。定量数据表明所研究的肌肉之间毛细血管尺寸存在差异。胸锁乳突肌白色部分的毛细血管最长，其DPP IV阳性段最短（占毛细血管总长度的8%）。比目鱼肌的毛细血管最短，其DPP IV阳性段也短（16%）。相比之下，胸锁乳突肌红色部分的DPP IV阳性段占毛细血管总长度的60%。横断面调查显示，经AP和DPP IV活性染色的毛细血管斑呈镶嵌分布。这项研究表明，在给定的肌纤维束内，与肌纤维平行的毛细血管彼此相对排列，使得它们的动脉段和静脉段对齐。
