Department of Microbiology, University of Delhi South Campus, New Delhi 110021, India.
Department of Microbiology, University of Delhi South Campus, New Delhi 110021, India.
Bioresour Technol. 2014;152:377-83. doi: 10.1016/j.biortech.2013.11.022. Epub 2013 Nov 19.
The present study deals with the development of an efficient ABE fermentation process using mixed substrate strategy for butanol production wherein no acetone was produced. For this, glucose was supplemented in the medium containing glycerol as main substrate which leads to a higher butanol production of 17.75 g/L in 72 h by Clostridium acetobutylicum KF158795. Moreover, the high cell inoculum also resulted in an increased ABE productivity of 0.46 g/L/h. Further, industrial scalability of the process was also successfully validated in a 300 L fermenter. Furthermore, potential of the Polymeric (PolyRMem) and Zeolite (ZeoMem) membranes for separation of butanol from fermentation broth was also studied by testing the pervaporation performance through which the butanol was successfully recovered.
本研究采用混合基质策略开发了一种高效的 ABE 发酵工艺，用于生产丁醇，且过程中不产生丙酮。为此，在含有甘油作为主要基质的培养基中补充葡萄糖，这使得丙酮丁醇梭菌 KF158795 在 72 小时内生产出 17.75 g/L 的丁醇。此外，高细胞接种量也使 ABE 的生产力提高到 0.46 g/L/h。此外，该工艺的工业放大规模也在 300 L 发酵罐中得到了成功验证。此外，还通过渗透蒸发性能测试研究了聚合物（PolyRMem）和沸石（ZeoMem）膜从发酵液中分离丁醇的潜力，成功回收了丁醇。
