The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-8580, Japan.
Faculty of Fisheries, Kagoshima University, 4-50-20 Shimoarata, Kagoshima 890-0056, Japan.
Fish Shellfish Immunol. 2014 Jan;36(1):299-304. doi: 10.1016/j.fsi.2013.11.016. Epub 2013 Dec 4.
Edwardsiella tarda is an intracellular pathogen that causes edwardsiellosis in fish. Our previous study suggests that cell-mediated immunity (CMI) plays an essential role in protection against E. tarda infection. In the present study, we adoptively transferred T-cell subsets sensitized with E. tarda to isogenic naïve ginbuna crucian carp to determination the T-cell subsets involved in protecting fish from E. tarda infection. Recipients of CD4(+) and CD8α(+) cells acquired significant resistance to infection with E. tarda 8 days after sensitization, indicating that helper T cells and cytotoxic T lymphocytes plays crucial roles in protective immunity to E. tarda. Moreover, transfer of sensitized CD8α(+) cells up-regulated the expression of genes encoding interferon-γ (IFN-γ) and perforin, suggesting that protective immunity to E. tarda involves cell-mediated cytotoxicity and interferon-γ-mediated induction of CMI. The results establish that CMI plays a crucial role in immunity against E. tarda. These findings provide novel insights into understanding the role of CMI to intracellular pathogens of fish.
迟缓爱德华氏菌是一种胞内病原体，可引起鱼类爱德华氏菌病。我们之前的研究表明，细胞介导的免疫（CMI）在抵御迟缓爱德华氏菌感染中发挥着重要作用。在本研究中，我们将经迟缓爱德华氏菌致敏的 T 细胞亚群过继转移到同源的鲫鱼中，以确定参与保护鱼类免受迟缓爱德华氏菌感染的 T 细胞亚群。致敏后 8 天，接受 CD4(+)和 CD8α(+)细胞的受体获得了对迟缓爱德华氏菌感染的显著抗性，表明辅助性 T 细胞和细胞毒性 T 淋巴细胞在迟缓爱德华氏菌的保护性免疫中发挥着关键作用。此外，过继转移致敏的 CD8α(+)细胞上调了编码干扰素-γ（IFN-γ）和穿孔素的基因的表达，表明针对迟缓爱德华氏菌的保护性免疫涉及细胞介导的细胞毒性和 IFN-γ 介导的 CMI 诱导。这些结果确立了 CMI 在抵御迟缓爱德华氏菌中的关键作用。这些发现为理解细胞介导的免疫对鱼类胞内病原体的作用提供了新的见解。
