Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2014 Mar 25;122:198-203. doi: 10.1016/j.saa.2013.11.075. Epub 2013 Nov 20.
This report presents a highly sensitive, poly(A)-stabilized gold nanoparticle-based assay with dual readouts (resonance light scattering and colorimetric) for detecting palmatine hydrochloride (PaH) in real samples. The detection mechanism is based on the fact that palmatine hydrochloride has strong affinity to poly(A), which can stabilize gold nanoparticles at high ionic strength, and cause the aggregation of poly(A)-stabilized AuNPs, resulting in the enhanced resonance light scattering (RLS). At the same time, the color change of poly(A)-stabilized AuNPs solution is from red to blue via purple. Thus a highly sensitive RLS assay for PaH has been developed with a linear range of 0.023-2.5 μg/mL. The limit of detection (LOD, 3σ) is 2.3 ng/mL. In this work, the reaction mechanism of this system was investigated by scanning electron microscope (SEM), dark-field light scattering images (DLSI), dynamiclight scattering (DLS) and circular dichroism (CD). This proposed method was also applied successfully for the determination of PaH in pharmaceutical preparations and urine samples with RSD⩽4.0%. The results are in good agreement with those from the official method.
本报告介绍了一种基于金纳米粒子的高灵敏、多聚腺苷酸稳定的双重读出（共振光散射和比色）分析方法，用于检测实际样品中的盐酸巴马汀（PaH）。检测机制基于盐酸巴马汀与多聚腺苷酸具有很强的亲和力，这种亲和力可以在高离子强度下稳定金纳米粒子，并导致多聚腺苷酸稳定的 AuNPs 聚集，从而增强共振光散射（RLS）。同时，多聚腺苷酸稳定的 AuNPs 溶液的颜色从红色变为紫色再变为蓝色。因此，开发了一种高灵敏度的 RLS 测定 PaH 的方法，其线性范围为 0.023-2.5μg/mL。检测限（LOD，3σ）为 2.3ng/mL。在这项工作中，通过扫描电子显微镜（SEM）、暗场光散射图像（DLSI）、动态光散射（DLS）和圆二色性（CD）研究了该体系的反应机制。该方法还成功地用于测定药物制剂和尿液样品中的 PaH，相对标准偏差（RSD）⩽4.0%。结果与官方方法一致。
