Ding Sheng, Zhou Ruiqing, Zhu Yaqin
Department of General Dentistry, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, People's Republic of China.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Dec;69(Pt 12):1371-3. doi: 10.1107/S1744309113029230. Epub 2013 Nov 29.
LKB1, a tumour suppressor, plays key roles in cell polarity, cell growth and energy metabolism. 14-3-3 proteins bind to LKB1 and suppress its functions. A chimera containing 14-3-3ζ and its binding region in LKB1 was constructed and the chimeric protein LKB1-14-4-3ζ was purified and crystallized. The crystal of LKB1-14-4-3ζ diffracted to 2.9 Å resolution and belonged to space group R32, with unit-cell parameters a = b = 130.262, c = 264.960 Å. Structure determination and refinement are in progress.
肿瘤抑制因子LKB1在细胞极性、细胞生长和能量代谢中发挥关键作用。14-3-3蛋白与LKB1结合并抑制其功能。构建了一种包含14-3-3ζ及其在LKB1中的结合区域的嵌合体，并纯化和结晶了嵌合蛋白LKB1-14-4-3ζ。LKB1-14-4-3ζ的晶体衍射分辨率达到2.9 Å，属于R32空间群，晶胞参数a = b = 130.262，c = 264.960 Å。结构测定和优化正在进行中。
