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烟曲霉（R）-选择性胺转氨酶的结晶及初步X射线衍射研究。

Crystallization and preliminary X-ray diffraction studies of the (R)-selective amine transaminase from Aspergillus fumigatus.

作者信息

Thomsen Maren, Skalden Lilly, Palm Gottfried J, Höhne Matthias, Bornscheuer Uwe T, Hinrichs Winfried

机构信息

Institut für Biochemie, Universität Greifswald, Felix-Hausdorff-Strasse 4, D-17489 Greifswald, Germany.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Dec;69(Pt 12):1415-7. doi: 10.1107/S1744309113030923. Epub 2013 Nov 29.

DOI:10.1107/S1744309113030923
PMID:24316843
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3855733/
Abstract

The (R)-selective amine transaminase from Aspergillus fumigatus was expressed in Escherichia coli and purified to homogeneity. Bright yellow crystals appeared while storing the concentrated solution in the refrigerator and belonged to space group C222(1). X-ray diffraction data were collected to 1.27 Å resolution, as well as an anomalous data set to 1.84 Å resolution that was suitable for S-SAD phasing.

摘要

来自烟曲霉的（R）-选择性胺转氨酶在大肠杆菌中表达并纯化至同质。将浓缩溶液储存在冰箱中时出现亮黄色晶体，其属于空间群C222(1)。收集了分辨率为1.27 Å的X射线衍射数据，以及分辨率为1.84 Å的反常数据集，该反常数据集适用于单波长反常散射（S-SAD）相位分析。

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