Penman George A, Lockhart Deborah E A, Ferenbach Andrew, van Aalten Daan M F
Division of Molecular Microbiology, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jun 1;68(Pt 6):705-8. doi: 10.1107/S1744309112017915. Epub 2012 May 23.
Aspergillus fumigatus UDP-galactopyranose mutase (AfUGM) is a potential drug target involved in the synthesis of the cell wall of this fungal pathogen. AfUGM was recombinantly produced in Escherichia coli, purified and crystallized by the sitting-drop method, producing orthorhombic crystals that diffracted to a resolution of 3.25 Å. The crystals contained four molecules per asymmetric unit and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 127.72, b = 134.30, c = 173.84 Å. Incorporation of selenomethionine was achieved, but the resulting crystals did not allow solution of the phase problem.
烟曲霉UDP-吡喃半乳糖变位酶（AfUGM）是这种真菌病原体细胞壁合成过程中一个潜在的药物靶点。AfUGM在大肠杆菌中进行重组表达，经纯化后采用坐滴法进行结晶，得到了正交晶体，其衍射分辨率达到3.25 Å。每个不对称单元的晶体包含四个分子，属于空间群P2(1)2(1)2(1)，晶胞参数为a = 127.72、b = 134.30、c = 173.84 Å。实现了硒代甲硫氨酸的掺入，但所得晶体无法解决相位问题。
