Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany.
Inflamm Res. 2014 Mar;63(3):239-47. doi: 10.1007/s00011-013-0694-0. Epub 2013 Dec 8.
Expression and function of histamine H4-receptor, an immunomodulatory receptor involved in inflammatory diseases, on murine macrophages, which are vital for immunity, were investigated.
The expression pattern of histamine receptors on bone marrow-derived macrophages of BALB/c mice and on RAW 264.7 cells was studied at the mRNA level by reverse transcription polymerase chain reaction. The functional relevance of histamine receptors was investigated by analyzing histamine-induced chemotaxis and phagocytosis in the presence of histamine receptor antagonists mepyramine (histamine H1-receptor), famotidine (histamine H2-receptor), thioperamide (histamine H3/4-receptors) and JNJ7777120 (histamine H4-receptor).
Both bone marrow-derived macrophages and RAW 264.7 cells express mRNA for histamine H1-receptor and histamine H4-receptor. Residual amounts of histamine H2-receptor mRNA are found in bone marrow-derived macrophages only. In both cellular models, histamine induced chemotaxis and phagocytic activity, which was reduced by thioperamide as well as by JNJ 7777120, but not by mepyramine or famotidine.
In murine bone marrow-derived macrophages and RAW 264.7 macrophage-like cells histamine H4-receptor mediates chemotaxis and phagocytic activity.
研究参与炎症性疾病的免疫调节受体组胺 H4 受体在对免疫至关重要的鼠源巨噬细胞中的表达和功能。
通过逆转录聚合酶链反应在 mRNA 水平研究 BALB/c 小鼠骨髓来源的巨噬细胞和 RAW 264.7 细胞上组胺受体的表达模式。通过分析组胺受体拮抗剂甲哌氯丙嗪（组胺 H1 受体）、法莫替丁（组胺 H2 受体）、噻哌酰胺（组胺 H3/4 受体）和 JNJ7777120（组胺 H4 受体）存在时组胺诱导的趋化性和吞噬作用，研究组胺受体的功能相关性。
骨髓来源的巨噬细胞和 RAW 264.7 细胞均表达组胺 H1 受体和组胺 H4 受体的 mRNA。仅在骨髓来源的巨噬细胞中发现组胺 H2 受体 mRNA 的残留量。在这两种细胞模型中，组胺诱导趋化性和吞噬活性，该活性可被噻哌酰胺和 JNJ7777120 降低，但不能被甲哌氯丙嗪或法莫替丁降低。
在鼠源骨髓来源的巨噬细胞和 RAW 264.7 巨噬细胞样细胞中，组胺 H4 受体介导趋化性和吞噬活性。
