Institute of Pharmacology, Hannover Medical School, Hannover, Germany.
J Interferon Cytokine Res. 2012 Apr;32(4):185-90. doi: 10.1089/jir.2011.0082. Epub 2012 Jan 26.
Histamine is involved in the execution of an immune reaction. Receptors for histamine, of which four different subtypes are known so far, are found on dendritic cells and on T cells. Via these receptors, histamine either indirectly or directly affects the activation of T cells. Data in the literature regarding the involved receptor subtypes and the mode of action of histamine on T cells are somewhat contradictory and depend on the type of cells analyzed, polarized T cells, or freshly prepared T cells within the context of the whole splenocyte population. Therefore, we analyzed the effect of histamine on murine T cells within splenocytes in a detailed manner. We stimulated freshly prepared splenocytes in the presence or absence of histamine with α-CD3 in vitro and analyzed the induced cytokine production. We show that histamine reduced the α-CD3-induced interferon-γ (IFN-γ) production of CD4⁺ cells via the histamine H₂-receptor. Moreover, the effect of histamine on the α-CD3-induced IFN-γ production could be transferred within conditioned splenocyte supernatants induced by histamine (in the absence of α-CD3). Thus, the histamine effect is mediated by a soluble factor, which, however, is neither of the classical anti-inflammatory mediators, interleukin-10, or transforming growth factor-β.
组胺参与免疫反应的执行。目前已知有四种不同亚型的组胺受体存在于树突状细胞和 T 细胞上。通过这些受体，组胺可以间接或直接影响 T 细胞的激活。文献中的数据表明，涉及的受体亚型以及组胺对 T 细胞的作用方式有些矛盾，这取决于所分析的细胞类型、极化的 T 细胞或在整个脾细胞群体中新鲜制备的 T 细胞。因此，我们详细分析了组胺对脾细胞中鼠 T 细胞的影响。我们在体外用 α-CD3 刺激新鲜制备的脾细胞，有或没有组胺，并分析诱导的细胞因子产生。我们表明，组胺通过组胺 H₂受体降低了 CD4⁺细胞中 α-CD3 诱导的干扰素-γ（IFN-γ）产生。此外，组胺对 α-CD3 诱导的 IFN-γ产生的影响可以通过组胺诱导的条件性脾细胞上清液（在没有 α-CD3 的情况下）传递。因此，组胺的作用是由一种可溶性因子介导的，然而，这种因子既不是经典的抗炎介质白细胞介素-10 也不是转化生长因子-β。
