de Mesquita Harleson Lopes, de Carvalho Giuliano Reder, Aarestrup Fernando Monteiro, Corrêa José Otávio do Amaral, Azevedo Maria Regina Andrade
Rev Bras Reumatol. 2013 Sep-Oct;53(5):400-4.
The antiphospholipid syndrome (APS) is an autoimmune condition characterized by recurrent arterial and venous thrombosis, besides obstetric complications. The pathogenesis is associated with the presence of antiphospholipid and/or anti-b2-glicoprotein I (anti-b2GPI) antibodies that appear to change the anticoagulant activity of b2GPI. Antibody-induced dimerization of b2GPI seems to be related to the induction of platelet aggregation, contributing to the development of thrombosis in APS.
The objective of the present study is to demonstrate the influence of antiphospholipid antibodies in platelet aggregation tests with different agonists (ADP, collagen, and adrenaline).
We analyzed platelet aggregation tests with different agonists (ADP, collagen, adrenalin) when normal platelets were exposed to serum with different concentrations of antiphospholipid antibodies.
Results demonstrated a significant inhibition in adrenalin- and ADP-induced platelet aggregation curves (P < 0.05) in all antibody concentrations tested when compared to the control. The paradox between the prothrombotic state and the presence of autoantibodies that show anticoagulant activity in vitro was demonstrated in the literature, making it difficult to understand the pathophysiologic mechanism of the antiphospholipid syndrome.
Results showed that anticardiolipin and anti-b2GPI antibodies-rich serum, both of which belonging to the IgG class, can interfere with platelet aggregation curves.
抗磷脂综合征（APS）是一种自身免疫性疾病，其特征除产科并发症外，还包括反复发生的动脉和静脉血栓形成。发病机制与抗磷脂和/或抗β2糖蛋白I（抗β2GPI）抗体的存在有关，这些抗体似乎会改变β2GPI的抗凝活性。抗体诱导的β2GPI二聚化似乎与血小板聚集的诱导有关，促进了APS中血栓形成的发展。
本研究的目的是证明抗磷脂抗体在不同激动剂（ADP、胶原和肾上腺素）的血小板聚集试验中的影响。
当正常血小板暴露于不同浓度抗磷脂抗体的血清时，我们分析了不同激动剂（ADP、胶原、肾上腺素）的血小板聚集试验。
结果表明，与对照组相比，在所有测试的抗体浓度下，肾上腺素和ADP诱导的血小板聚集曲线均有显著抑制（P < 0.05）。文献中证实了血栓前状态与体外具有抗凝活性的自身抗体的存在之间的矛盾，这使得理解抗磷脂综合征的病理生理机制变得困难。
结果表明，富含抗心磷脂和抗β2GPI抗体的血清（均属于IgG类）可干扰血小板聚集曲线。
