Hirabayashi Masumi, Goto Teppei, Tamura Chihiro, Sanbo Makoto, Hara Hiromasa, Hochi Shinichi
Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Aichi 444-8787, Japan.
J Reprod Dev. 2014 Mar 7;60(1):78-82. doi: 10.1262/jrd.2013-109. Epub 2013 Dec 7.
This study was designed to investigate whether supplementation of 2i medium with leukemia inhibitory factor (LIF) and/or forskolin would support establishment of germline-competent rat embryonic stem (ES) cell lines. Due to the higher likelihood of outgrowth rates, supplementation of forskolin with or without LIF contributed to the higher establishment efficiency of ES cell lines in the WDB strain. Germline transmission competency of the chimeric rats was not influenced by the profile of ES cell lines until their establishment. When the LIF/forskolin-supplemented 2i medium was used, the rat strain used as the blastocyst donor, such as the WI strain, was a possible factor negatively influencing the establishment efficiency of ES cell lines. Once ES cell lines were established, all lines were found to be germline-competent by a progeny test in chimeric rats. In conclusion, both LIF and forskolin are not essential but can play a beneficial role in the establishment of "genuine" rat ES cell lines.
本研究旨在调查在2i培养基中添加白血病抑制因子（LIF）和/或福司可林是否有助于建立具有种系传递能力的大鼠胚胎干细胞（ES）系。由于生长率较高的可能性，添加或不添加LIF的福司可林有助于提高WDB品系中ES细胞系的建立效率。在嵌合大鼠中，直到ES细胞系建立，其种系传递能力不受ES细胞系特征的影响。当使用添加LIF/福司可林的2i培养基时，用作囊胚供体的大鼠品系，如WI品系，可能是对ES细胞系建立效率产生负面影响的一个因素。一旦建立了ES细胞系，通过对嵌合大鼠的后代测试发现所有细胞系都具有种系传递能力。总之，LIF和福司可林都不是必需的，但在建立“真正的”大鼠ES细胞系中可以发挥有益作用。
