集胞藻PCC 6803中活性羰基的清除系统
Scavenging systems for reactive carbonyls in the cyanobacterium Synechocystis sp. PCC 6803.
作者信息
Shimakawa Ginga, Suzuki Mayumi, Yamamoto Eriko, Nishi Akiko, Saito Ryota, Sakamoto Katsuhiko, Yamamoto Hiroshi, Makino Amane, Miyake Chikahiro
机构信息
Department of Biological and Environmental Sciences, Faculty of Agriculture, Graduate School of Agricultural Science, Kobe University.
出版信息
Biosci Biotechnol Biochem. 2013;77(12):2441-8. doi: 10.1271/bbb.130554. Epub 2013 Dec 7.
To elucidate the scavenging systems of sugar- and lipid-derived reactive carbonyls (RCs) in the cyanobacterium Synechocystis sp. PCC 6803 (S. 6803), we selected proteins from S. 6803 based on amino-acid (AA) sequence similarities with proteins from Arabidopsis thaliana, and characterized the properties of the GST-fusion proteins expressed. Slr0942 catalyzed the aldo-keto reductase (AKR) reaction scavenging mainly sugar-derived RCs, methylglyoxal (MG). Slr1192 is the medium-chain dehydrogenase/redutase (MDR). It catalyzed the AKR reaction scavenging several lipid-derived RCs, acrolein, propionaldehyde, and crotonaldehyde. Slr0315 is a short-chain dehydrogenase/redutase (SDR), and it catalyzed only the reduction of MG in the AKR reaction. Slr0381 catalyzed the conversion of hemithioacetal to S-lactoylglutahione (SLG) in the glyoxalase (GLX) 1 reaction. Sll1019 catalyzed the conversion of SLG to glutathione and lactate in the GLX2 reaction. GLX1 and GLX2 compose the glyoxalase system, which scavenges MG. These enzymes contribute to scavenging sugar- and lipid-derived RCs as scavenging systems.
为了阐明集胞藻6803（S. 6803）中糖和脂质衍生的活性羰基化合物（RCs）的清除系统，我们基于与拟南芥蛋白质的氨基酸（AA）序列相似性从S. 6803中选择蛋白质，并对所表达的谷胱甘肽S-转移酶（GST）融合蛋白的特性进行了表征。Slr0942催化醛酮还原酶（AKR）反应，主要清除糖衍生的RCs，即甲基乙二醛（MG）。Slr1192是中链脱氢酶/还原酶（MDR）。它催化AKR反应，清除几种脂质衍生的RCs，即丙烯醛、丙醛和巴豆醛。Slr0315是一种短链脱氢酶/还原酶（SDR），它在AKR反应中仅催化MG的还原。Slr0381在乙二醛酶（GLX）1反应中催化半硫代乙缩醛转化为S-乳酰谷胱甘肽（SLG）。Sll1019在GLX2反应中催化SLG转化为谷胱甘肽和乳酸。GLX1和GLX2组成乙二醛酶系统，该系统可清除MG。这些酶作为清除系统，有助于清除糖和脂质衍生的RCs。
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