Gao Liang, Garcia-Uribe Alejandro, Liu Yan, Li Chiye, Wang Lihong V
Department of Biomedical Engineering, Washington University in St Louis, One Brookings Dr., St Louis, MO 63130, USA.
J Cell Sci. 2014 Jan 15;127(Pt 2):288-94. doi: 10.1242/jcs.142943. Epub 2013 Dec 6.
We present a generic sub-diffraction-limited imaging method - photobleaching imprinting microscopy (PIM) - for biological fluorescence imaging. A lateral resolution of 110 nm was measured, more than a twofold improvement over the optical diffraction limit. Unlike other super-resolution imaging techniques, PIM does not require complicated illumination modules or specific fluorescent dyes. PIM is expected to facilitate the conversion of super-resolution imaging into a routine lab tool, making it accessible to a much broader biological research community. Moreover, we show that PIM can increase the image contrast of biological tissue, effectively extending the fundamental depth limit of multi-photon fluorescence microscopy.
我们提出了一种用于生物荧光成像的通用亚衍射极限成像方法——光漂白印记显微镜（PIM）。测量得到横向分辨率为110纳米，比光学衍射极限提高了两倍多。与其他超分辨率成像技术不同，PIM不需要复杂的照明模块或特定的荧光染料。预计PIM将有助于将超分辨率成像转化为常规实验室工具，使更广泛的生物研究群体能够使用。此外，我们表明PIM可以提高生物组织的图像对比度，有效扩展多光子荧光显微镜的基本深度极限。
