Univ Franche - Comté, 25000, Besançon, France.
Anal Bioanal Chem. 2014 Jan;406(3):905-9. doi: 10.1007/s00216-013-7532-7. Epub 2013 Dec 7.
A new carbon nanotube porous silica poroshell stationary phase was developed. The chromatographic support was coated with ultrashort single-wall carbon nanotubes (SWCNTs) in a noncovalent way. It was demonstrated that the porous amino silica surface of the 300 NH2 poroshell column stabilized with 1-methyl-2-pyrrolidinone efficiently and stably adsorbed SWCNTs onto the chromatographic support. It was shown that this novel poroshell carbon nanotube (CNT) stationary phase was very useful for the HPLC separation of a series of monoclonal antibodies (mAbs) in a short analysis time (<3 min). The high-performance liquid chromatography (HPLC) method was validated and was successfully tested for the fast quantitative and qualitative control of chemotherapeutic bags fabricated in a hospital pharmacy.
一种新型的碳纳米管多孔硅胶核壳固定相被开发出来。该色谱支撑体通过非共价方式涂覆了超短单壁碳纳米管（SWCNTs）。研究表明，300NH2 多孔氨基硅胶表面在 1-甲基-2-吡咯烷酮的稳定作用下，可将 SWCNTs 有效地、稳定地吸附到色谱支撑体上。研究表明，这种新型的核壳碳纳米管（CNT）固定相非常有助于在短分析时间（<3 分钟）内对一系列单克隆抗体（mAbs）进行高效液相色谱（HPLC）分离。该高效液相色谱法（HPLC）已得到验证，并成功地用于快速定量和定性控制医院药剂科制备的化疗袋。
