低温诱导鱼腥藻形态改变与其生长温度的关系。

Chill-induced morphological alterations in Anacystis Nidulans as a function of growth temperature.

机构信息

Department of Botany and Cell Research Institute, University of Texas, 78712, Austin, TX, USA.

出版信息

Planta. 1979 Jan;145(1):63-8. doi: 10.1007/BF00379928.

Abstract

Cells of Anacystis nidulans grown at 25 or 30°C were examined both by thin-section and freeze-fracture electron microscopy. Cells grown at either temperature appeared similar when fixed at the growth temperature prior to observation. When cells were chilled to near 0°C for 30 min prior to fixation, those previously grown at 25° appeared unchanged as judged by thin sectioning while those grown at 39° showed considerable morphological alteration. Freeze fracture showed particle aggregation (more pronounced in 39°-grown cells) indicating lipid-phase separation in cells chilled prior to fixation. The phase separation was totally reversed by rewarming the chilled, 25°-grown cells to their growth temperature but was only partially reversed by rewarming chilled, 39°-grown cells. These results correlate with other effects of chilling seen in Anacystis cells grown at different temperatures.

摘要

在 25°C 或 30°C 下生长的鱼腥藻细胞分别通过薄切片和冷冻断裂电子显微镜进行了检查。在观察之前，在生长温度下固定时，在两种温度下生长的细胞看起来相似。当细胞在固定前冷却到接近 0°C 30 分钟时，通过薄切片判断，以前在 25°C 下生长的细胞没有变化，而在 39°C 下生长的细胞显示出相当大的形态改变。冷冻断裂显示颗粒聚集（在 39°C 生长的细胞中更为明显），表明在固定前冷却的细胞中出现脂质相分离。相分离在冷却的 25°C 生长的细胞被重新加热到其生长温度时完全逆转，但在冷却的 39°C 生长的细胞中仅部分逆转。这些结果与在不同温度下生长的鱼腥藻细胞中观察到的其他冷却效应相关。