Brand J J
Department of Botany, The University of Texas at Austin, Austin, Texas 78712.
Plant Physiol. 1977 May;59(5):970-3. doi: 10.1104/pp.59.5.970.
When Anacystis nidulans, strain TX 20 was grown at 39 C, then rapidly chilled to 0 C, a pigment with a carotenoid-like spectrum was bleached. This effect was not seen when cells which had been grown at 25 C were chilled. The effect seen in 39 C-grown cells was not reversible except under extreme conditions such as heating to near boiling for several minutes. Bleaching could be prevented by prior exposure of cells to glutaraldehyde, but could not be reversed by glutaraldehyde treatment following chilling. The effect occurred upon chilling 39 C-grown cells even after extensive heating at 85 C, a treatment which destroys phycocyanin and metabolic activities. 25 C-grown cells were induced to bleach by chilling when suspended in 50% glycerol. The results are interpreted as indicating a chill-induced change in aggregation state of a carotenoid, which changes its specific absorbance.
当聚球藻菌株TX 20在39℃下生长，然后迅速冷却至0℃时，一种具有类胡萝卜素光谱的色素会被漂白。当在25℃下生长的细胞被冷却时，不会出现这种效应。在39℃下生长的细胞中观察到的这种效应是不可逆的，除非在极端条件下，如加热至接近沸腾几分钟。细胞预先暴露于戊二醛可防止漂白，但冷却后用戊二醛处理不能使其恢复。即使在85℃下长时间加热（这种处理会破坏藻蓝蛋白和代谢活性）后，将39℃下生长的细胞冷却时仍会出现这种效应。当25℃下生长的细胞悬浮在50%甘油中时，冷却会诱导其漂白。结果被解释为表明冷却诱导了类胡萝卜素聚集状态的变化，从而改变了其特定吸光度。
