一种测定核酮糖二磷酸羧化酶/加氧酶 CO2/O2 特异性因子的简易方法。
A facile method to determine the CO2/O 2 specificity factor for ribulose bisphosphate carboxylase/oxygenase.
机构信息
Department of Biochemistry and Biophysics, Washington State University, 99164-4660, Pullman, WA, USA.
出版信息
Photosynth Res. 1993 Jul;37(1):81-6. doi: 10.1007/BF02185441.
A rapid method to determine the CO2/O2 specificity factor of ribulose 1,5-bisphosphate carboxylase/oxygenase is presented. The assay measures the amount of CO2 and O2 fixation at varying CO2/O2 ratios to determine the relative rates of each reaction. CO2 fixation is measured by the incorporation of the moles of(14)CO2 into 3-phosphoglycerate, while O2 fixation is determined by subtraction of the moles of CO2 fixed from the moles of RuBP consumed in each reaction. By analyzing the inorganic phosphate specifically hydrolyzed from RuBP under alkaline conditions, the amount of RuBP present before and after catalysis by rubisco can be determined.
本文介绍了一种快速测定核酮糖 1,5-二磷酸羧化酶/加氧酶的 CO2/O2 专一性因子的方法。该测定法通过测定不同 CO2/O2 比例下 CO2 和 O2 的固定量来确定每个反应的相对速率，从而测量 CO2 的固定量。CO2 的固定量通过将(14)CO2 掺入 3-磷酸甘油酸中的摩尔数来测量，而 O2 的固定量则通过从每个反应中 RuBP 消耗的 CO2 摩尔数中减去 CO2 的固定量来确定。通过在碱性条件下分析 RuBP 特异性水解产生的无机磷，可以确定 Rubisco 催化前后 RuBP 的含量。
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