Regeneration of vascular tissue in wounded pea roots.

Severance of the stele of young main roots of pea (Pisum sativum L.) results in formation of a bridge of vascular tissue in the remaining cortex. Cell divisions occur close to the severed vascular tissues on both the proximal and distal sides of the cut within 24 h. Differentiation of new vascular strands subsequently begins in the same locations and progresses from both sides of the wound into the remaining cortex and also back along the original vascular strands. Most of the vascular tissue which forms the bridge through the cortex differentiates in the acropetal direction. Continuous strands composed of single sieve elements bypass the wound somewhat sooner than the first complete xylem strands; the latter in 60-70% of the cases, are present by 3 d. Cambial activity subsequently adds more xylem and phloem. Vascular regeneration is not affected by removal of the epicotyl or the root tip; it is greatly reduced but not prevented by removal of the cotyledons.