Biology Department, Queen Elizabeth College, Campden Hill Road, W8 7AH, London, UK.
Planta. 1983 Jul;157(4):307-16. doi: 10.1007/BF00397401.
Quantitative cytochemical studies of cortical parenchyma cells of roots of Pisum sativum in which the central vascular bundle is severed, showed esterase activity to be an early marker of the determination of cells to form a vascular bridge. Explantation, onto a basal culture medium, of wound segments taken from roots at different times after severing the stele showed the irreversibility of the esterase activity on removal from the inducing environment, so confirming this as a marker of cell determination. A general determination for the stele was shown to occur by 8-10 h after wounding, but information relating to tracheid secondary-cell-wall formation was not apparently available until 18-20 h after wounding. Determination appeared to occur well before mitosis. The timings of the differentiation steps indicate a simple diffusion model to explain the mechanism of arrival of the initiating molecules.
对被切断中心维管束的豌豆根皮层细胞的定量细胞化学研究表明，酯酶活性是细胞决定形成维管束桥的早期标志。将不同时间从切断中柱的根上取下的伤口段外植到基础培养基上，显示出在除去诱导环境后，酯酶活性的不可逆性，从而证实这是细胞决定的标志。在受伤后 8-10 小时显示出对中柱的一般决定，但直到受伤后 18-20 小时才出现与木质部次生细胞壁形成有关的信息。决定似乎发生在有丝分裂之前。分化步骤的时间表明，一个简单的扩散模型可以用来解释起始分子到达的机制。
