Dudits D, Hadlaczky G, Lévi E, Fejér O, Haydu Z, Lázár G
Insitute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary.
Theor Appl Genet. 1977 May;51(3):127-32. doi: 10.1007/BF00273824.
Protoplasts isolated from cultured cells of albino carrot (Daucus carota) and normal green D. capillifolius were fused by polyethylene glycol. Selection of somatic hybrid plants was based on the restoration of photosynthetic function in hybrids. Green plantlets selected from embryo cultures were characterized on the basis of leaf morphology. The interspecific protoplast fusion resulted in green plants with leaves which were intermediate between those of the parents. The somatic hybrids between orange rooted carrot variety and D. capillifolius with long white roots produced long, white and fleshy roots. The cytological analysis of parasexual hybrids revealed that the chromosome number ranged from 34 to 54. The most frequent chromosome number was 2n = 36. Hybrids were also found with 34 and 35 chromosomes. The somatic hybrid showed the same isoenzyme pattern of leaf peroxidase as D. carota.
从白化胡萝卜（胡萝卜属）和正常绿色的细叶胡萝卜的培养细胞中分离出的原生质体，通过聚乙二醇进行融合。体细胞杂种植物的选择基于杂种中光合功能的恢复。从胚培养物中选出的绿色小植株根据叶片形态进行了鉴定。种间原生质体融合产生了绿色植株，其叶片形态介于双亲之间。橙色根胡萝卜品种与长白色根的细叶胡萝卜之间的体细胞杂种产生了长的、白色且肉质的根。准性杂种的细胞学分析表明，染色体数范围为34至54。最常见的染色体数为2n = 36。还发现有染色体数为34和35的杂种。体细胞杂种显示出与胡萝卜相同的叶片过氧化物酶同工酶模式。
