Erwin-Baur-Institut, Abteilung Straub, Max-Planck-Institut für Züchtungsforschung, D-5000, Köln 30, Federal Republic of Germany.
Planta. 1979 Jan;145(4):371-5. doi: 10.1007/BF00388363.
After fusion of protoplasts from a diploid (2n=24) and a tetraploid (4n=48) chlorophyll-deficient mutant of Datura innoxia Mill. with diploid (2n=72) green wild-type protoplasts of Atropa belladonna L. thirteen somatic hybrids could be selected, most of which had already started to produce leaves and shoots. Hybrid calli were recognizable by the production of hairs, typical for Datura innoxia, and the green colour, derived from Atropa belladonna. Further proof for the hybrid nature was furnished by cytological investigations. The metaphase chromosomes of both species are easily distinguishable in their size: chromosomes of Datura innoxia are about twice as large as those of Atropa belladonna. The chromosome numbers of the hybrids varied from ca. 84 to ca. 175.
二倍体（2n=24）和四倍体（4n=48）叶绿素缺失突变体矮牵牛原生质体融合后，与颠茄（Atropa belladonna L.）二倍体（2n=72）绿色野生型原生质体融合，可选择出 13 个体细胞杂种，其中大多数已经开始产生叶片和芽。杂种愈伤组织通过产生典型的矮牵牛的毛发和绿色颜色来识别，这是由颠茄衍生而来的。细胞学研究进一步证明了杂种的性质。这两个物种的中期染色体在大小上很容易区分：矮牵牛的染色体大约是颠茄的两倍大。杂种的染色体数从约 84 到约 175 不等。
