Department of Genetics, Moscow State University, 119899, Moscow, Russian Federation.
Photosynth Res. 1993 Aug;37(2):139-46. doi: 10.1007/BF02187472.
Ten strains from a collection of mutants ofSynechocystis 6803 defective in Photosystem II (PS II) function were transformed with chromosomal DNA of wild-type and mutant cells. Cross hybridization data allowed to identify four groups of PS II-mutants. Highly efficient transformation was observed between different mutant groups, but not within the groups. Restoration of photosynthetic activity of the mutant cells was also achieved by transformation with different parts of a 5.6 kbBam HI fragment of wild typeSynechocystis DNA containing thepsbB gene. Each group of mutants was transformed to photoautotrophic growth by specific subfragments of thepsbB gene. DNA fragments of four selected mutant strains hybridizing with thepsbB gene were isolated and sequenced. The mutations were identified as a single nucleotide insertion or substitution leading to stop codon formation in two of the mutants, as a deletion of 12 nucleotides, or as a nucleotide substitution resulting in an amino acid substitution in the other two mutants. Deletion of 12 nucleotides in mutant strain PMB1 and stop codon formation in strain NF16 affect membrane-spanning regions of the gene product, the CP 47 protein.
从集光器 II（PS II）功能缺陷的集胞藻 6803 突变体中挑选出 10 株菌，用野生型和突变型细胞的染色体 DNA 进行转化。通过杂交数据鉴定，这些 PS II 突变体可分为 4 组。不同突变体组之间观察到高效转化，但在组内没有观察到转化。用含有 psbB 基因的 5.6kbBam HI 片段的不同部分转化突变细胞，也可以恢复其光合活性。每种突变体组都可以通过 psbB 基因的特定亚片段转化为光自养生长。与 psbB 基因杂交的 4 个选定的突变株的 DNA 片段被分离并测序。突变被鉴定为单个核苷酸插入或取代，导致两个突变株中的终止密码子形成，另两个突变株中 12 个核苷酸缺失或核苷酸取代导致氨基酸取代。突变株 PMB1 中的 12 个核苷酸缺失和 NF16 株中的终止密码子形成影响基因产物的跨膜区域，即 CP 47 蛋白。
