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玉米(Zea mays L.)完整组织和分离组织中的胚胎蛋白相同。

Identical embryonal proteins in intact and isolated tissues of maize (Zea mays L.).

机构信息

Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Academy of Sciences of USSR, P.O. Box 1243, 664033, Irkutsk 33, USSR.

出版信息

Planta. 1979 Jan;145(3):245-51. doi: 10.1007/BF00454448.

Abstract

Minor antigens characteristic of developing and mature embryos were not found in the shoot and root meristems of the seedlings. Some of these embryonal antigens (EA) were present, however, in callus and cell-suspension cultures, irrespective of their tissue origin, and were maintained throughout repeated subcultures, in some cases for more than 2 years. These EA were distinct both from the meristematic antigens found in the intact seedlings and in callus cultures, and from organ-specific antigens found only in intact plants. The EA of callus tissues derived from several maize genotypes were serologically identical. We therefore assume that these EA are "proliferation proteins" or "early proteins" expressed by cells that have not undergone any determination and lack any tissue or organ specificity.

摘要

在幼苗的茎尖和根分生组织中未发现发育和成熟胚胎所特有的次要抗原。然而,在愈伤组织和细胞悬浮培养物中存在一些胚胎抗原(EA),无论其组织来源如何,并且在多次继代培养中都能保持,在某些情况下超过 2 年。这些 EA 既不同于完整幼苗中发现的分生组织抗原,也不同于仅在完整植物中发现的器官特异性抗原。来自几种玉米基因型的愈伤组织的 EA 在血清学上是相同的。因此,我们假设这些 EA 是未经历任何决定且缺乏任何组织或器官特异性的细胞表达的“增殖蛋白”或“早期蛋白”。

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